Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro

Yan Lei; Qian Gao; Ke-ji Chen

doi:10.1007/s11655-008-0037-0

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Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro

Updated：2021-08-27

- Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro
- Chinese Journal of Integrative Medicine Vol. 14, Issue 1, Pages: 37-41(2008)
- Affiliations：
  
  1. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Shijia Research Center, Peking University,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. A30070956)
- DOI：10.1007/s11655-008-0037-0
  CLC：
- Published：2008，
  
  Published Online：21 June 2008，
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Lei, Y., Gao, Q. & Chen, Kj. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro, ., Chin. J. Integr. Med. 14, 37–41 (2008). https://doi.org/10.1007/s11655-008-0037-0

Yan Lei, Qian Gao, Ke-ji Chen. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro. [J]. Chinese Journal of Integrative Medicine 14(1):37-41(2008)
Lei, Y., Gao, Q. & Chen, Kj. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro, ., Chin. J. Integr. Med. 14, 37–41 (2008). https://doi.org/10.1007/s11655-008-0037-0 DOI：

Yan Lei, Qian Gao, Ke-ji Chen. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro. [J]. Chinese Journal of Integrative Medicine 14(1):37-41(2008) DOI： 10.1007/s11655-008-0037-0.

摘要

To study the effects of extracts from Panax notoginseng (EPN) and Panax ginseng fruit (EPGF) on the proliferation and migration of human umbilical vein endothelial cells (HUVECs) in vitro. Cell proliferation was determined using an MTT method with a cultured HUVECs model cell cycle analyzed by cytometry. The effect on endothelial cell migration was investigated using an agarose scraping method. The content of vascular endothelial growth factor (VEGF) in the supernate was determined by enzyme-linked immunosorbent assay (ELISA). The VEGF mRNA expression of vascular endothelial cells (VECs) with different concentrations of EPN and EPGF was examined by reverse transcriptase-polymerase chain reaction (RT-PCR). EPN and EPGF can promote the proliferation of VECs and the secretion of VEGF from HUVECs. It can increase the cell population significantly in the S phase to (15.22±1.33) % in the 50 mg/L dose group (P<0.05 or P<0.01). They can promote the VEC migration in the 200 mg/L dose group and the migration rate was 93.75% (P<0.01). They could also increase VEGF mRNA expression in VEC and the effects in the 100 mg/L and 50 mg/L dose groups were signifi cant with the proportion of VEGF mRNA expression of 0.1812±0.0413 and 0.2037±0.0399 respectively (P<0.01). EPN and EPGF can promote VEC proliferation

migration

DNA synthesis and VEGF mRNA expression. The results suggest that they have a certain effect on the genesis and development of new vessels in the ischemic myocardium.

Abstract

migration

DNA synthesis and VEGF mRNA expression. The results suggest that they have a certain effect on the genesis and development of new vessels in the ischemic myocardium.

关键词

endothelial cellvascular endothelial growth factorproliferationmigration

Keywords

endothelial cellvascular endothelial growth factorproliferationmigration

references

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Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

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