Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells

Shu-sheng Li; Jun Feng; Zhi Zheng; Qian-sheng Liang

doi:10.1007/s11655-008-0123-3

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Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells

Updated：2021-08-27

- Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells
- Chinese Journal of Integrative Medicine Vol. 14, Issue 2, Pages: 123-127(2008)
- Affiliations：
  
  Department of Emergency Internal Medicine, Tongji Hospital, Huazhong University of Science and Technology,Wuhan,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-008-0123-3
  CLC：
- Published：2008，
  
  Published Online：6 August 2008，
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Li, Ss., Feng, J., Zheng, Z. et al. Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells., Chin. J. Integr. Med. 14, 123–127 (2008). https://doi.org/10.1007/s11655-008-0123-3

Shu-sheng Li, Jun Feng, Zhi Zheng, et al. Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells. [J]. Chinese Journal of Integrative Medicine 14(2):123-127(2008)
Li, Ss., Feng, J., Zheng, Z. et al. Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells., Chin. J. Integr. Med. 14, 123–127 (2008). https://doi.org/10.1007/s11655-008-0123-3 DOI：

Shu-sheng Li, Jun Feng, Zhi Zheng, et al. Effect of sodium tanshinone II A sulfonate on phosphorylation of extracellular signal-regulated kinase 1/2 in angiotensin II-induced hypertrophy of myocardial cells. [J]. Chinese Journal of Integrative Medicine 14(2):123-127(2008) DOI： 10.1007/s11655-008-0123-3.

摘要

To observe the effects of sodium tanshinone II A sulfonate (STS) on angiotensin II (Ang II)-induced hypertrophy of myocardial cells through the expression of phosphorylated extracellular signal-regulated kinase (p-ERK1/2). In the primary culture of neonatal rat myocardial cells

the total protein content in myocardial cells was determined by coomassie brilliant blue and the protein synthesis rate was measured by [3H]-Leucine incorporation as indexes for hypertrophy of myocardial cells. The expression of p-ERK1/2 was determined using Western blot and immunofluorescence labeling. (1) The total protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial cells were stimulated by Ang II (1 μmol/L) for 24 h; STS markedly inhibited the increment of the total protein level induced by Ang II and the syntheses of protein. (2) After pretreatment of myocardial cells with Ang II (1 μmol/L) for 5 min

the p-ERK1/2 protein expression was increased

with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with STS at different doses (2

50 μmol/L) for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated by Ang II in a dose-dependent manner. (3) After the myocardial cells were stimulated by Ang II (1 μmol/L)

the immunofl uorescence of ERK1/2 rapidly appeared in the nucleus. The activation and translocation process of ERK1/2 induced by Ang II was blocked distinctly by STS. STS inhibited the myocardial cell hypertrophy induced by Ang II

and the mechanism may be associated with the inhibition of p-ERK1/2 expression.

Abstract

the p-ERK1/2 protein expression was increased

with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with STS at different doses (2

and the mechanism may be associated with the inhibition of p-ERK1/2 expression.

关键词

sodium tanshinone II A sulfonateangiotensin IImyocardial cell hypertrophyextracellular signal-regulated kinase

Keywords

sodium tanshinone II A sulfonateangiotensin IImyocardial cell hypertrophyextracellular signal-regulated kinase

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Department of Hematology, the Affiliated Hospital of Guiyang Medical College

Key Laboratory of Basic Pharmacology of Guizhou and Department of Pharmacology, Zunyi Medical College

The Experimental Research Center, China Academy of Chinese Medical Sciences

The Cardiology Department of Chongqing Hospital of Traditional Chinese Medicine and Chongqing the First Hospital

Department of Radioisotope, Qilu Hospital Affiliated to Shandong University

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