Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro

Hui Liao; Linda K. Banbury; David N. Leach

doi:10.1007/s11655-008-0128-y

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Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro

Updated：2021-08-27

- Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro
- Chinese Journal of Integrative Medicine Vol. 14, Issue 2, Pages: 128-131(2008)
- Affiliations：
  
  1. Shanxi Provincial People’s Hospital,Taiyuan,China
  2. Centre for Phytochemistry and Pharmacology, Southern Cross University, Lismore,Australia
- Author bio：
- Funds：
- DOI：10.1007/s11655-008-0128-y
  CLC：
- Published：2008，
  
  Published Online：6 August 2008，
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Liao, H., Banbury, L.K. & Leach, D.N. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro, ., Chin. J. Integr. Med. 14, 128–131 (2008). https://doi.org/10.1007/s11655-008-0128-y

Hui Liao, Linda K. Banbury, David N. Leach. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro. [J]. Chinese Journal of Integrative Medicine 14(2):128-131(2008)
Liao, H., Banbury, L.K. & Leach, D.N. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro, ., Chin. J. Integr. Med. 14, 128–131 (2008). https://doi.org/10.1007/s11655-008-0128-y DOI：

Hui Liao, Linda K. Banbury, David N. Leach. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro. [J]. Chinese Journal of Integrative Medicine 14(2):128-131(2008) DOI： 10.1007/s11655-008-0128-y.

摘要

To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill (丹栀逍遥丸

DXP) on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC). The effects of DXP on nitric oxide (NO) production were tested by lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages using the Griess reaction. The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10

0.25 and 0.50 mg/mL dose-dependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820 μmoL Trolox equivalent/g

about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL (P<0.05

P<0.01). The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells

with the effect possibly related to its antioxidant activity and the inhibition of NO production.

Abstract

To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill (丹栀逍遥丸

0.25 and 0.50 mg/mL dose-dependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820 μmoL Trolox equivalent/g

about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL (P<0.05

P<0.01). The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells

with the effect possibly related to its antioxidant activity and the inhibition of NO production.

关键词

Danzhi Xiaoyao Pillbreast cancer MCF-7 cellsantioxidant activityoxygen radical absorbance capacityNitric Oxide

Keywords

Danzhi Xiaoyao Pillbreast cancer MCF-7 cellsantioxidant activityoxygen radical absorbance capacityNitric Oxide

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Department of Hematology, the Affiliated Hospital of Guiyang Medical College

Key Laboratory of Basic Pharmacology of Guizhou and Department of Pharmacology, Zunyi Medical College

Department of Physiology, Bengbu Medical College

Tianjin University of Traditional Chinese Medicine

The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine

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