Relationship between platelet activation related factors and polymorphism of related genes in patients with coronary heart disease of blood-stasis syndrome

Mei Xue; Ke-ji Chen; Hui-jun Yin

doi:10.1007/s11655-008-0267-1

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Relationship between platelet activation related factors and polymorphism of related genes in patients with coronary heart disease of blood-stasis syndrome

Updated：2021-08-27

- Relationship between platelet activation related factors and polymorphism of related genes in patients with coronary heart disease of blood-stasis syndrome
- Chinese Journal of Integrative Medicine Vol. 14, Issue 4, Pages: 267-273(2008)
- Affiliations：
  
  Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Major Program Project of National Natural Science Foundation of China (No. 90409021)
- DOI：10.1007/s11655-008-0267-1
  CLC：
- Published：2008，
  
  Published Online：12 December 2008，
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Xue, M., Chen, Kj. & Yin, Hj. Relationship between platelet activation related factors and polymorphism of related genes in patients with coronary heart disease of blood-stasis syndrome., Chin. J. Integr. Med. 14, 267–273 (2008). https://doi.org/10.1007/s11655-008-0267-1

Mei Xue, Ke-ji Chen, Hui-jun Yin. Relationship between platelet activation related factors and polymorphism of related genes in patients with coronary heart disease of blood-stasis syndrome. [J]. Chinese Journal of Integrative Medicine 14(4):267-273(2008)
Xue, M., Chen, Kj. & Yin, Hj. Relationship between platelet activation related factors and polymorphism of related genes in patients with coronary heart disease of blood-stasis syndrome., Chin. J. Integr. Med. 14, 267–273 (2008). https://doi.org/10.1007/s11655-008-0267-1 DOI：

Mei Xue, Ke-ji Chen, Hui-jun Yin. Relationship between platelet activation related factors and polymorphism of related genes in patients with coronary heart disease of blood-stasis syndrome. [J]. Chinese Journal of Integrative Medicine 14(4):267-273(2008) DOI： 10.1007/s11655-008-0267-1.

摘要

To comparatively study the expressive conditions of platelet activation related factors (GP I b

GP II b- III a and GMP-140) in healthy subjects and patients with coronary heart disease (CHD) of blood-stasis (BS) or non-blood-stasis (non-BS) syndrome

and to analyze the relationship between the activities of various glycoproteins and the polymorphism of genes. With case control design adopted

patients with the CHD (40 of BS

37 of non-BS) and 39 healthy subjects for control

all fitting to the inclusion criteria

were selected in this study. The number of affected coronary branches was recorded by the contrast examination. The mean fluorescence intensity (MFI) of GP I b

GP II b- III a

and GMP-140 (CD42b

CD61

CD62p) in patients and healthy persons was measured with flow cytometry

the polymorphism of HPA-3 gene was detected by Taqman probe technique and that of HPA-2 gene was determined by gene sequencing. MFI of CD61 and CD62p was higher in the CHD patients than in the healthy control

which was also higher in patients of BS syndrome than in patients of non-BS syndrome (P<0.05); MFI of CD42b was lower in the CHD patients than in the healthy control (P<0.05)

but showing insignificant difference between BS and non-BS syndrome (P>0.05); at the same time

no significant difference of all the above-mentioned three MFI could be found in patients with various numbers of affected coronary branches

neither in patients with different genotypes at GP II b HPA-3 and GP I b HPA-2 polymorphism loci (P>0.05). (1) The activities of GP II b- III a and GMP-140 were obviously increased in the genesis and developing process of CHD and CHD of BS syndrome

and so they could be taken as one of the objective indexes for microscopic diagnosis of BS syndrome. (2) The level of GP I b was lower in CHD patients than in healthy persons

but it was not a sensitive indicator for BS syndrome of CHD. (3) Levels of GP II b- III a

GP I b and GMP-140 were not related with the number of affected coronary branches in CHD patients. (4) The changes in amino-acids expression induced by the two loci brought no significant influence on GP I b and GP II b- III a activities.

Abstract

To comparatively study the expressive conditions of platelet activation related factors (GP I b

GP II b- III a and GMP-140) in healthy subjects and patients with coronary heart disease (CHD) of blood-stasis (BS) or non-blood-stasis (non-BS) syndrome

and to analyze the relationship between the activities of various glycoproteins and the polymorphism of genes. With case control design adopted

patients with the CHD (40 of BS

37 of non-BS) and 39 healthy subjects for control

all fitting to the inclusion criteria

were selected in this study. The number of affected coronary branches was recorded by the contrast examination. The mean fluorescence intensity (MFI) of GP I b

GP II b- III a

and GMP-140 (CD42b

CD61

CD62p) in patients and healthy persons was measured with flow cytometry

which was also higher in patients of BS syndrome than in patients of non-BS syndrome (P<0.05); MFI of CD42b was lower in the CHD patients than in the healthy control (P<0.05)

but showing insignificant difference between BS and non-BS syndrome (P>0.05); at the same time

no significant difference of all the above-mentioned three MFI could be found in patients with various numbers of affected coronary branches

and so they could be taken as one of the objective indexes for microscopic diagnosis of BS syndrome. (2) The level of GP I b was lower in CHD patients than in healthy persons

but it was not a sensitive indicator for BS syndrome of CHD. (3) Levels of GP II b- III a

关键词

coronary heart diseaseblood-stasis syndromeGP II b- III aGP I bGMP-140platelet activationgene polymorphism

Keywords

coronary heart diseaseblood-stasis syndromeGP II b- III aGP I bGMP-140platelet activationgene polymorphism

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Department of Cardiovascular Disease, Xiyuan Hospital, China Academy of Chinese Medical Sciences

Department of Cardiovascular Disease, Xiyuan Hospital, China Academy of Traditional Chinese Medicine

College of Health Information Technology and Management, Shanghai University of Medicine and Health Sciences

Department of Cardiologic Medicine, The Second People’s Hospital of Fujian Province

The Second Clinic of Chinese Medicine, Fujian University of Traditional Chinese Medicine

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