Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium

Ling Qu; Xiao-chun Liang; Hong Zhang; Qun-li Wu; Lian-qing Sun; Bei Gu

doi:10.1007/s11655-008-0293-z

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Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium

Updated：2021-08-27

- Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium
- Chinese Journal of Integrative Medicine Vol. 14, Issue 4, Pages: 293-297(2008)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences,Beijing,China
  2. Cell Center, Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of China (No. 30572438)
- DOI：10.1007/s11655-008-0293-z
  CLC：
- Published：2008，
  
  Published Online：12 December 2008，
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Qu, L., Liang, Xc., Zhang, H. et al. Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium., Chin. J. Integr. Med. 14, 293–297 (2008). https://doi.org/10.1007/s11655-008-0293-z

Ling Qu, Xiao-chun Liang, Hong Zhang, et al. Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium. [J]. Chinese Journal of Integrative Medicine 14(4):293-297(2008)
Qu, L., Liang, Xc., Zhang, H. et al. Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium., Chin. J. Integr. Med. 14, 293–297 (2008). https://doi.org/10.1007/s11655-008-0293-z DOI：

Ling Qu, Xiao-chun Liang, Hong Zhang, et al. Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium. [J]. Chinese Journal of Integrative Medicine 14(4):293-297(2008) DOI： 10.1007/s11655-008-0293-z.

摘要

To investigate the effect of Jinmaitong (筋脉通

JMT) serum on the proliferation of rat Schwann cells (SCs) primarily cultured in high glucose medium. SCs were primarily cultured in Dulbecco’s minmum essential medium (DMEM control)

50 mmol/L glucose medium (50 mmol/L Glu)

75 mmol/L glucose medium (75 mmol/L Glu)

as well as 50 mmol/L glucose medium

with different concentrations of JMT serum (undiluted

1:2 diluted and 1:8 diluted) and Neurotropin (Ntp)

respectively. The proliferation of SCs under different conditions was detected by MTT. SCs grew exuberantly in DMEM within 24–72 h

but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48

72 and 96 h

which showed that both were significantly different compared to the control group (P<0.01). The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu (P<0.05). Spearman’s rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose (r=−0.471

P<0.01). Excluding the time factor

partial correlation showed similar results (r=−0.679

P<0.01). After 48 h

the proliferation of SCs increased significantly in JMT1:2 and Ntp compared with 50 mmol/L Glu (control 0.437±0.019

50 mmol/L Glu 0.367±0.035

JMT1:2 0.426±0.024

Ntp 0.422±0.013; P<0.01)

and there were no statistically significant differences among the JMT groups

the Ntp group and the control group (P>0.05). The proliferation of SCs was inhibited in high glucose medium

and the inhibition was reduced by different concentrations of JMT serum

especially at JMT1:2.

Abstract

To investigate the effect of Jinmaitong (筋脉通

JMT) serum on the proliferation of rat Schwann cells (SCs) primarily cultured in high glucose medium. SCs were primarily cultured in Dulbecco’s minmum essential medium (DMEM control)

50 mmol/L glucose medium (50 mmol/L Glu)

75 mmol/L glucose medium (75 mmol/L Glu)

as well as 50 mmol/L glucose medium

with different concentrations of JMT serum (undiluted

1:2 diluted and 1:8 diluted) and Neurotropin (Ntp)

respectively. The proliferation of SCs under different conditions was detected by MTT. SCs grew exuberantly in DMEM within 24–72 h

but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48

72 and 96 h

P<0.01). Excluding the time factor

partial correlation showed similar results (r=−0.679

P<0.01). After 48 h

the proliferation of SCs increased significantly in JMT1:2 and Ntp compared with 50 mmol/L Glu (control 0.437±0.019

50 mmol/L Glu 0.367±0.035

JMT1:2 0.426±0.024

Ntp 0.422±0.013; P<0.01)

and there were no statistically significant differences among the JMT groups

the Ntp group and the control group (P>0.05). The proliferation of SCs was inhibited in high glucose medium

and the inhibition was reduced by different concentrations of JMT serum

especially at JMT1:2.

关键词

Schwann cellshigh glucoseprimary cultureJinmaitongproliferationChinese herbs’ serum pharmacology

Keywords

Schwann cellshigh glucoseprimary cultureJinmaitongproliferationChinese herbs’ serum pharmacology

references

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Liang XC, Cui LY, Guo SS, et al. Clinical study on Jinmaitong composita on diabetic peripheral neuropathy. Chin J Integr Tradit West Med (Chin) 1999;19(9):517–519.

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Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences

Department of Electron Microscope, Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences

Chinese Medicine Hospital of Shanxi Province

Shanghai Key Laboratory of Complex Prescription

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