Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene

Xiao-juan Ma; Hui-jun Yin; Ke-ji Chen

doi:10.1007/s11655-009-0101-4

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Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene

Updated：2021-08-27

- Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene
- Chinese Journal of Integrative Medicine Vol. 15, Issue 2, Pages: 101-106(2009)
- Affiliations：
  
  Department of Cardiovascular Disease, Xiyuan Hospital, China Academy of Traditional Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Major Research Plan of National Natural Science Foundation of China (No. 90409021)
- DOI：10.1007/s11655-009-0101-4
  CLC：
- Published：2009，
  
  Published Online：29 April 2009，
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Ma, Xj., Yin, Hj. & Chen, Kj. Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene., Chin. J. Integr. Med. 15, 101–106 (2009). https://doi.org/10.1007/s11655-009-0101-4

Xiao-juan Ma, Hui-jun Yin, Ke-ji Chen. Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene. [J]. Chinese Journal of Integrative Medicine 15(2):101-106(2009)
Ma, Xj., Yin, Hj. & Chen, Kj. Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene., Chin. J. Integr. Med. 15, 101–106 (2009). https://doi.org/10.1007/s11655-009-0101-4 DOI：

Xiao-juan Ma, Hui-jun Yin, Ke-ji Chen. Differential gene expression profiles in coronary heart disease patients of blood stasis syndrome in traditional Chinese medicine and clinical role of target gene. [J]. Chinese Journal of Integrative Medicine 15(2):101-106(2009) DOI： 10.1007/s11655-009-0101-4.

摘要

To investigate the differential gene expression profiles in coronary heart disease (CHD) patients of blood-stasis syndrome (BSS) by oligonucleotide microarray technique

and the clinical significance of target gene. Subjects were assigned to CHD patients with BSS (n=8)

CHD patients without BSS (n=8)

and BSS patients without CHD (n=8) based on coronary angiography and the diagnostic criteria of BSS. The sex- and age-matched healthy volunteers (n=8) were enrolled as the control group. Venous blood samples were collected for RNA extraction; Test-3 chip was employed to examine the quality of samples. Then

the samples were hybridized with Affymetrix U133 Plus 2.0 array to compare the gene expression profiles among the four groups. Gene-array scanner and gene chip operating software were applied to screen out hybridization signals and analyze gene expression

respectively. Based on the comparison of the samples of the four groups

the differential genes related with CHD and BSS were analyzed with Gene Ontology (GO) and pathway

and target genes selected were confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR). Thirty CHD patients with BSS were selected according to the former criteria and 40 healthy as controls. The serum concentration of interleukin-8 (IL-8) was determined by double-antibody sandwich avidin-biotin peroxidase complex enzyme-linked (ABC-ELISA). A total of 107 differential genes were found being associated with CHD

including 48 up-regulated genes and 59 down-regulated genes. Among these 107 differential genes

14 genes (13.1%) were found related to inflammatory reaction and immune response through GO analysis. In the pathway analysis

4 of 15 conspicuous pathways were referred to the inflammation and immune response. Among 48 differential genes related to BSS

26 genes were up-regulated

and 22 were down-regulated. Five of the 48 genes (10.4%) and 5 of 10 signficant pathways were involved in inflammation and immunity. The results of real-time RT-PCR proved the accuracy of the gene chip. The patients have markedly higher level of serum IL-8 compared to the controls (P<0.05). The correlation of inflammatory- and immune-related genes with CHD patients of BSS was revealed at the level of nucleic acid

and the target gene IL-8 may play a role in the pathobiology of CHD with BSS.

Abstract

To investigate the differential gene expression profiles in coronary heart disease (CHD) patients of blood-stasis syndrome (BSS) by oligonucleotide microarray technique

and the clinical significance of target gene. Subjects were assigned to CHD patients with BSS (n=8)

CHD patients without BSS (n=8)

respectively. Based on the comparison of the samples of the four groups

the differential genes related with CHD and BSS were analyzed with Gene Ontology (GO) and pathway

including 48 up-regulated genes and 59 down-regulated genes. Among these 107 differential genes

14 genes (13.1%) were found related to inflammatory reaction and immune response through GO analysis. In the pathway analysis

4 of 15 conspicuous pathways were referred to the inflammation and immune response. Among 48 differential genes related to BSS

26 genes were up-regulated

and the target gene IL-8 may play a role in the pathobiology of CHD with BSS.

关键词

coronary heart diseaseblood-stasis syndromedifferential gene expression profiletarget gene

Keywords

coronary heart diseaseblood-stasis syndromedifferential gene expression profiletarget gene

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Xiyuan Hospital, China Academy of Chinese Medical Sciences

Cardiovascular Diseases Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences

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Department of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences

Academy of Chinese Medical Sciences

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