Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance

Huang Jia-dong; Song Zhao; Li Jing; Wu Bao-yan; Yin Hui-jun; Chen Ke-ji; Chen Qiang

doi:10.1007/BF02835747

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Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance

OriginalPaper | Updated：2021-08-27

- Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance
- Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance
- 中国结合医学杂志（英文版） 2005年11卷第1期页码：37-40
- Affiliations：
  
  1. College of Life Sciences, Nankai University,Tianjin
  2. Xiyuan Hospital, China Academy of Traditional Chinese Medicine,China
- Author bio：
- Funds：
  
  Supported by the National Nature Science Foundation of China,(No. 90209054)
- DOI：10.1007/BF02835747
  中图分类号：
- 纸质出版日期：2005，
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Jia-dong, H., Zhao, S., Jing, L. et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance., Chin. J. Integr. Med. 11, 37–40 (2005). https://doi.org/10.1007/BF02835747

Huang Jia-dong, Song Zhao, Li Jing, et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance[J]. Chinese Journal of Integrative Medicine, 2005,11(1):37-40.
Jia-dong, H., Zhao, S., Jing, L. et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance., Chin. J. Integr. Med. 11, 37–40 (2005). https://doi.org/10.1007/BF02835747 DOI：

Huang Jia-dong, Song Zhao, Li Jing, et al. Study on the therapeutic mechanism of the active principle of the Chinese drug Paeoniae Radix 801 through affinity biosensors IAsys Plus quartz crystal microbalance[J]. Chinese Journal of Integrative Medicine, 2005,11(1):37-40. DOI： 10.1007/BF02835747.

摘要

Objective: To study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs

Paeoniae Radix 801(PR801) in its cardiovascular protective effects and its specific binding with endothelin 1(ET-1) as well as the dynamics of the two’s interactive function by means of using affinity biosensors: IAsys Plus and quartz crystal microbalance (IAQCM).Methods: ET-1 was immobilized on the surfaces of IAQCM by using the new surface modification methods. The PR801 in the solution was detected by modified substrates and the specific binding between PR801 and ET-1 was studied.Results: The curves went up or down after adding PR801. There is specific binding between PR801 and ET-1. The bound mass were 0.458 ng/mm2 and 133. 54 ng/cm2

respectively. There exists relatively good stability with these two methods.Conclusion: The affinity biosensors: IAQCM can be used to study the interaction mechanism between PR801 and ET-1

providing a new way to study the interaction mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore

ET-1 is another target that PR801 can bind specifically besides thromboxane A2.

Abstract

Objective: To study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs

respectively. There exists relatively good stability with these two methods.Conclusion: The affinity biosensors: IAQCM can be used to study the interaction mechanism between PR801 and ET-1

providing a new way to study the interaction mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore

ET-1 is another target that PR801 can bind specifically besides thromboxane A2.

关键词

activating blood circulation to remove stasisPaeoniae Radix 801endothelin-1affinity biosensors

Keywords

activating blood circulation to remove stasisPaeoniae Radix 801endothelin-1affinity biosensors

references

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