An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro

Han Jin; Zhao Yan-ling; Shan Li-mei; Huang Feng-jiao; Xiao Xiao-he

doi:10.1007/BF02835751

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An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro

OriginalPaper | Updated：2021-08-27

- An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro
- An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro
- 中国结合医学杂志（英文版） 2005年11卷第1期页码：54-56
- Affiliations：
  
  1. Department of Pharmacy, Hospital of PLA,Beijing,302
  2. Department of Pharmacy, Hubei Traditional Chinese Medical College,Wuhan
- Author bio：
- Funds：
  
  Supported by the Project of Military Science during the 9th Five-Year Plan Period (No. 01G19) and the National Natural Science Foundation (No. 39970911)
- DOI：10.1007/BF02835751
  中图分类号：
- 纸质出版日期：2005，
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Jin, H., Yan-ling, Z., Li-mei, S. et al. An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro, ., Chin. J. Integr. Med. 11, 54–56 (2005). https://doi.org/10.1007/BF02835751

Han Jin, Zhao Yan-ling, Shan Li-mei, et al. An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro[J]. Chinese Journal of Integrative Medicine, 2005,11(1):54-56.
Jin, H., Yan-ling, Z., Li-mei, S. et al. An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro, ., Chin. J. Integr. Med. 11, 54–56 (2005). https://doi.org/10.1007/BF02835751 DOI：

Han Jin, Zhao Yan-ling, Shan Li-mei, et al. An experiment on standardized cell culture assay in assessing the activities of Composite Artemisia Capillaris Tablets against hepatitis B virus replicationin vitro[J]. Chinese Journal of Integrative Medicine, 2005,11(1):54-56. DOI： 10.1007/BF02835751.

摘要

Objective: To explore the activities of Composite Artemisia Capillaris Tablet (CACT) against hepatitis B virus replicationin vitro.Methods: By means of radioimmunoassay (RIA)

Dot blot and Southern blot

the surface and e antigen production of 2.2.15 cells

HBV DNA in 2.2.15 cell culture medium and that in 2.2.15 cells were examined respectively.Results: HBsAg

HBeAg values of 2.2.15 cells treated by CACT were lower than those of the control

the HBV DNA quantities in culture medium and in 2.2. 15 cells decreased as compared with those cells with no treatment by CACT given to them.Conclusion: CACT could inhibit HBV DNA replication

showing its potential antiviral activity in hepatitis B treatment.

Abstract

Objective: To explore the activities of Composite Artemisia Capillaris Tablet (CACT) against hepatitis B virus replicationin vitro.Methods: By means of radioimmunoassay (RIA)

Dot blot and Southern blot

the surface and e antigen production of 2.2.15 cells

HBV DNA in 2.2.15 cell culture medium and that in 2.2.15 cells were examined respectively.Results: HBsAg

HBeAg values of 2.2.15 cells treated by CACT were lower than those of the control

the HBV DNA quantities in culture medium and in 2.2. 15 cells decreased as compared with those cells with no treatment by CACT given to them.Conclusion: CACT could inhibit HBV DNA replication

showing its potential antiviral activity in hepatitis B treatment.

关键词

Composite Artemisia Capillaris Tablet2.2.15 cellssurface antigene antigenHBV DNA

Keywords

Composite Artemisia Capillaris Tablet2.2.15 cellssurface antigene antigenHBV DNA

references

Korba BE, Milman. A cell culture assay for compounds which inhibit hepatitis B virus replication. Antiviral Res 1991);15: 217–228.

Zhao YL, Yuan HL, Xiao XH, et al. Experimental study of the inhibitory effect of Gantai capsule on HBsAg and HBeAg expression. Chin J Integr Med 2002;8(1): 56–58.

Korba BE, Boyd MR. Penciclovir is a selective inhibitor of hepatitis B virus in cultured human hepatoblastoma cells. Antimicrob Agents Chemother 1996; 40: 1282–1284.

David E, Zembower, Yuh-Meei Lin, et al. Robustaflavone, a potential non-nucleoside anti-hepatitis B agent. Antiviral Res 1998;39: 81–88.

Belen’kii MS, Schinazi RF. Multiple drug effect analysis with confident interval. Antiviral Res 1994;25: 1–11.

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