Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo

Wang Jian; He Hui-juan; He Cheng-wei; Wu Ping; Liu Jian-jun

doi:10.1007/BF02836409

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Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo

OriginalPaper | Updated：2021-08-27

- Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo
- Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo
- 中国结合医学杂志（英文版） 2004年10卷第3期页码：208-212
- Affiliations：
  
  1. Affiliated Hospital to Guangdong Medical College,Zhanjiang,China
  2. Department of Biochemistry & Molecular Biology, Guangdong Medical College,Guangdong
- Author bio：
- Funds：
  
  The project was sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Personnels, State Education Ministry (Grant No. 247)
- DOI：10.1007/BF02836409
  中图分类号：
- 纸质出版日期：2004，
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Jian, W., Hui-juan, H., Cheng-wei, H. et al. Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo., Chin. J. Integr. Med. 10, 208–212 (2004). https://doi.org/10.1007/BF02836409

Wang Jian, He Hui-juan, He Cheng-wei, et al. Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo[J]. Chinese Journal of Integrative Medicine, 2004,10(3):208-212.
Jian, W., Hui-juan, H., Cheng-wei, H. et al. Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo., Chin. J. Integr. Med. 10, 208–212 (2004). https://doi.org/10.1007/BF02836409 DOI：

Wang Jian, He Hui-juan, He Cheng-wei, et al. Effect of Allicin in antagonizing mice’s bladder cancer in vitro and in vivo[J]. Chinese Journal of Integrative Medicine, 2004,10(3):208-212. DOI： 10.1007/BF02836409.

摘要

Objective To explore anti-tumor effect and mechanism of Allicin in treating murine bladder tumor.Methods: To observe Allicin’s effect on MBT-2 tumor cells in vitro

100 ⧎g/ml Allicin was added to the tumor cell culture

and the morphology of tumor cells was observed by phase contrast microscope 6 hrs later. The direct effects of Allicin on tumor cell growth in vitro in the MTT Assay was also evaluated. To determine anti-tumor effect of Allicin in vivo

C3H/He mice were randomly grouped prior to initiation of experiment. The mice received 1 × 105 MBT-2 cells administered subcutaneously into the right posterior flank on the Day 0 the experiment started. Allicin was injected at the site near tumor transplantation on the Day 1. The mice were examined for tumor development and the tumors were measured in two dimensions with calipers twice a week. On Day 21 the tumors were resected and examined pathologically to see the immune response.Results: The observation of morphology of MBT-2 cells in vitro and MTT assay indicated that Allicin has apparent direct cytotoxicity to bladder cancer cells. In high dosage group

a marked delay was shown in the appearance and growth of tumors after subcutaneously injection when compared with the control group (P< 0.01). Histology studies suggested that there were more macrophages

lymphocytes and fibroblasts at the peri-tumor region than the control group.Conclusion: Allicin has a marked tumor inhibitory effect on bladder tumor. This effect could possibly be related to direct cytotoxicity and activation of immune response. It could as possibly prove to be an effective intravesical treatment agent for superficial bladder cancer.

Abstract

Objective To explore anti-tumor effect and mechanism of Allicin in treating murine bladder tumor.Methods: To observe Allicin’s effect on MBT-2 tumor cells in vitro

100 ⧎g/ml Allicin was added to the tumor cell culture

关键词

allicinanti-tumor effectBladder Tumor

Keywords

allicinanti-tumor effectBladder Tumor

references

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