The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage

Tang Tao; Li Xing-qun; Wu Heng; Luo Jie-kun; Zhang Hua-xian; Luo Tuan-lian

doi:10.1007/BF02836431

Your Location：

Home >

Browse articles >

The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage

OriginalPaper | Updated：2021-08-27

- The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage
- The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage
- 中国结合医学杂志（英文版） 2004年10卷第4期页码：284-288
- Affiliations：
  
  Institute of Integrative Traditional Chinese Medicine & Western Medicine, Xiangya Hospital, Central South University,Changsha
- Author bio：
- Funds：
  
  Project supported by the National Natural Science Foundation of China (No. 30171192).
- DOI：10.1007/BF02836431
  中图分类号：
- 纸质出版日期：2004，
Scan for full text
Tao, T., Xing-qun, L., Heng, W. et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage., Chin. J. Integr. Med. 10, 284–288 (2004). https://doi.org/10.1007/BF02836431

Tang Tao, Li Xing-qun, Wu Heng, et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage[J]. Chinese Journal of Integrative Medicine, 2004,10(4):284-288.
Tao, T., Xing-qun, L., Heng, W. et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage., Chin. J. Integr. Med. 10, 284–288 (2004). https://doi.org/10.1007/BF02836431 DOI：

Tang Tao, Li Xing-qun, Wu Heng, et al. The Property of Nao Yi-an granule in increasing Nestin immunoreactive neural stem cells in rat brains with intracerebral hemorrhage[J]. Chinese Journal of Integrative Medicine, 2004,10(4):284-288. DOI： 10.1007/BF02836431.

摘要

Objective: To explore changes of neural stem cells (NSCs) following intracerebral hemorrhage (ICH) and the effect of Nao Yi-an granule (NYA) on them

and furthermore

to explore NYA’s therapeutic mechanismMethods: Sixty rats were randomly divided into three groups

the sham operation control group

the ICH group and the NYA-treated group. Except those in the sham operation control group

all other rats were replicated to ICH models by stereotactical injection of collagenase type VU into their globus pallidus. After modeling

the rats in the NYA-treated group were administered with NYA at the dosage of 4.92 g·kg-1·d-1 by gastrogavage

while those in the other two groups

4 ml distilled water were given instead. The behavioral test was used to evaluate neurological deficit and immunohistochemical method was used to detect Nestin expression

the special marker for neural stem cells.Results: The ICH animals showed notable placing deficit in forlimbs from day 1 to day 28 after modeling. The deficit in the NYA-treated group was similar to that of the ICH group

but the former got recovered better than the latter from day 21 to 28

with significant difference shown (P<0.05). Nestin-positive cells (N+ cells) could be seen around the hemotoma from day 2 on in the ICH group

the number of which gradually increased from day 4 to 7 (P< 0.01) and reached the peak on day 14 (P<0.05)

but reduced significantly on day 28 (P<0.01). Compared with that of the ICH group at the same time points

there was no significant difference in number of N+ cells in the NYA group on day 2 and 4

but it reached the peak earlier on day 7 (P<0.01)

with the level significantly higher than that in the ICH group (P<0.01)

and this high level lasted to day 14.Conclusion: NYA could promote the recovery of behavioral deficit in collagenase type VII-induced ICH rats

and increase the number of nestin-immunoreactive NSCs

suggesting that its effect on NSCs may be one of its pharmaceutical mechanisms in treating ICH.

Abstract

Objective: To explore changes of neural stem cells (NSCs) following intracerebral hemorrhage (ICH) and the effect of Nao Yi-an granule (NYA) on them

and furthermore

to explore NYA’s therapeutic mechanismMethods: Sixty rats were randomly divided into three groups

the sham operation control group

the ICH group and the NYA-treated group. Except those in the sham operation control group

all other rats were replicated to ICH models by stereotactical injection of collagenase type VU into their globus pallidus. After modeling

the rats in the NYA-treated group were administered with NYA at the dosage of 4.92 g·kg-1·d-1 by gastrogavage

while those in the other two groups

4 ml distilled water were given instead. The behavioral test was used to evaluate neurological deficit and immunohistochemical method was used to detect Nestin expression

but the former got recovered better than the latter from day 21 to 28

with significant difference shown (P<0.05). Nestin-positive cells (N+ cells) could be seen around the hemotoma from day 2 on in the ICH group

the number of which gradually increased from day 4 to 7 (P< 0.01) and reached the peak on day 14 (P<0.05)

but reduced significantly on day 28 (P<0.01). Compared with that of the ICH group at the same time points

there was no significant difference in number of N+ cells in the NYA group on day 2 and 4

but it reached the peak earlier on day 7 (P<0.01)

with the level significantly higher than that in the ICH group (P<0.01)

and this high level lasted to day 14.Conclusion: NYA could promote the recovery of behavioral deficit in collagenase type VII-induced ICH rats

and increase the number of nestin-immunoreactive NSCs

suggesting that its effect on NSCs may be one of its pharmaceutical mechanisms in treating ICH.

关键词

Nao Yi-an granuleneural stem cellintracerebral hemorrhagerats

Keywords

Nao Yi-an granuleneural stem cellintracerebral hemorrhagerats

references

Altman J and Das GD. Autoradiographic and histological evidence of postnatal neurogenesis in rats. J Comp Neurol 1965; 124: 319–335.

Reynolds BA and Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian nervous system. Science 1992; 255: 1707–1710.

Takagi Y, Nozaki K, Takahashi J, et al. Proliferation of neuronal precursor cells in dentate gyrus is accelerated after transient forebrain ischemia in mice. Brain Res 1999; 83(1–2): 283–287.

Tzeng SF, Wu JP. Responses of microglia and neural progenitor to mechanical brain injury. Neuroreport 1999; 10: 2887–2292.

Blumcke I, Schewe JC, Normann S, et al. Increase of nestin immunoreactive neural precursor cells in the dentate gyrus of pediatric patients with early-onset temporal epilepsy. Hippocampus 2001; 11: 311–321.

Cheng XM, Ziegler DK, Lai YHC, et al. Stroke in China, 1986 through 1990. Stroke 1995; 26: 1990–1994.

Zhen J, Li X, Jin Y. Effects of traditional Chinese medicine Nao Yi-An granule on the expression of HSP70 and NF-κB and apoptosis in ischemia cortex of rat brain after experimental hemorrhagic stroke. Chin J Basic Med Tradit Chin Med 2002; 8: 420–423.

Pen ZC, Li XQ, Liang QH, et al. Induction of NADPH-diaphorase activity in the forebrain in a model of intracerebral hemorrhage and its inhibition by the traditional Chinese medicine complex Nao Yi-an. Brain Res Bull 1997; 42: 119–128.

Zhang HB, Li XQ, Zhang MX, et al. Effects of Nao Yi-An on expression of bFGF in intracerebral hemorrhagic rat brains. Stroke Neurol Dis 2002; 8: 204–208.

Rosenberg GA, Mun-Bryce S, Wesley M, et al. Collagenase-induced intracerebral hemorrhage in rats. Stroke 1990; 21: 801–807.

Hua Y, Schallen T, Keep RF, et al. Behavioral tests after intracerebral hemorrhage in the rat. Stroke 2002; 33: 2478–2484.

Gage FH. Mammalian neural stem cells. 2000. Science 287: 1433–1438.

Kaya SS, Mahmood A, Li Y, et al. Expression of nestin after traumatic brain injury in rat brain. Brain Res 1999; 840: 153–157.

Lois C, Alvarez-Buylla A. Long-distance neuronal migration in the adult mammalian brain. Sci 1994; 264 (5162): 1145–1148.

Moskowitz MA, Lo EH. Neurogenesis and apoptosis cell death. Stroke 2003; 34: 324–326.

Prestoz L, Relvas JB, Hopkins K, et al. Association between integrin-dependent migration capacity of neural stem cellin vitro and anatomical repair following transplantation. Mol Cell Neurosci 2001; 18: 473–484.

Kim HM, Qu T, Kriho V, et al. Reelin function in neural stem cell biology. Proc Natl Acad Sci 2002; 99: 4020–4025.

Sato K, Hayashi T, Sasaki C, et al. Temporal and spatial differences of PSA-NCAM expression between young-adult and aged rats in normal and ischemic brains. Brain Res 2001; 922: 135–139.

Scott DE, Hansen SL. Post-traumatic regeneration, neurogenesis and neural migration in adult mammalian brain. Va Med Q 1997; 124: 249–261.

FULL TEXT LINK

More>

浏览量

418

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effect of two Chinese medicinal compounds, blood-activating and water-draining medicine, on tumor necrosis factor α and nuclear factor κ B expressions in rats with intracerebral hemorrhage

Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats

Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury

Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats

Scavenging effect of Naoerkang (脑尔康) on amyloid beta-peptide deposition in the hippocampus in a rat model of Alzheimer’s disease