Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast

Wang Cheng-xin; Huang Xuan; Zhao Xiao-ling; Deng Hao

doi:10.1007/BF02836468

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Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast

OriginalPaper | Updated：2021-08-27

- Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast
- Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast
- 中国结合医学杂志（英文版） 2005年11卷第2期页码：123-127
- Affiliations：
  
  1. Department of Pathology & Pathophysiology, School of Medicine and Life Sciences, Jianghan University,Wuhan
  2. Department of Pathology of Wuhan No. 4 Hospital, Jianghan University,China
- Author bio：
- Funds：
- DOI：10.1007/BF02836468
  中图分类号：
- 纸质出版日期：2005，
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Cheng-xin, W., Xuan, H., Xiao-ling, Z. et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast., Chin. J. Integr. Med. 11, 123–127 (2005). https://doi.org/10.1007/BF02836468

Wang Cheng-xin, Huang Xuan, Zhao Xiao-ling, et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast[J]. Chinese Journal of Integrative Medicine, 2005,11(2):123-127.
Cheng-xin, W., Xuan, H., Xiao-ling, Z. et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast., Chin. J. Integr. Med. 11, 123–127 (2005). https://doi.org/10.1007/BF02836468 DOI：

Wang Cheng-xin, Huang Xuan, Zhao Xiao-ling, et al. Comparative study of the effect of Shugan Shuru Granule on pathology and p53 gene expression in patients with hyperplastic disease of breast[J]. Chinese Journal of Integrative Medicine, 2005,11(2):123-127. DOI： 10.1007/BF02836468.

摘要

Objective: To study the effect of Shugan Shuru Granule (SSG) on the p53 gene expression in patients with hyperplastic disease of breast (HDB) to indirectly explore the mechanism of SSG’s effect on HDB on the molecular pathological level.Methods: Sixty-six patients with HDB were allocated in the treated group and the control group

with the former treated with SSG and the latter not. All patients underwent breast operation and their diseased mammary tissues were cut out

sectioned

and observed under microscopy with HE staining and immunohistochemical staining

with ABC method adopted to estimate the degree of hyperplasia and p53 gene expression. The severity of HDB was classified into normal

mild

moderate and severe grades (marked as 0 to III)

according to the degree of hyperplasia in the mammary gland.Results: Hyperplasia in the control group mostly belonged to grade I – III before treatment

showing overgrowth of gland and proliferation of glandular epithelial cells

which were high columnar shaped

more stratified

with papillary or substantive dysplasia. While in the treated group

most belonged to grade 0 – I after SSG treatment

with proliferated gland and dysplasia recovered to normal or disappeared. The positive rate of p53 gene expression in the treated group was 9.09%

and in the control group 39. 39%

comparison between the two groups showing significant difference (P<0. 01)

the intensity in the former was significantly weaker than that in the latter.Conclusion: SSG could not only inhibit the proliferation of mammary duct epithelia and lobuli

but also inhibit the over-expression of P53. Therefore

it could be regarded as an effective remedy for treatment of HDB and prevention of mammary cancer genesis.

Abstract

with the former treated with SSG and the latter not. All patients underwent breast operation and their diseased mammary tissues were cut out

sectioned

and observed under microscopy with HE staining and immunohistochemical staining

with ABC method adopted to estimate the degree of hyperplasia and p53 gene expression. The severity of HDB was classified into normal

mild

moderate and severe grades (marked as 0 to III)

according to the degree of hyperplasia in the mammary gland.Results: Hyperplasia in the control group mostly belonged to grade I – III before treatment

showing overgrowth of gland and proliferation of glandular epithelial cells

which were high columnar shaped

more stratified

with papillary or substantive dysplasia. While in the treated group

most belonged to grade 0 – I after SSG treatment

with proliferated gland and dysplasia recovered to normal or disappeared. The positive rate of p53 gene expression in the treated group was 9.09%

and in the control group 39. 39%

comparison between the two groups showing significant difference (P<0. 01)

the intensity in the former was significantly weaker than that in the latter.Conclusion: SSG could not only inhibit the proliferation of mammary duct epithelia and lobuli

but also inhibit the over-expression of P53. Therefore

it could be regarded as an effective remedy for treatment of HDB and prevention of mammary cancer genesis.

关键词

Shugan Shuru Granulehyperplastic disease of breastp53 gene

Keywords

Shugan Shuru Granulehyperplastic disease of breastp53 gene

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