Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection

Yu Yong-hao; Cui Nai-qiang; Fu Qiang; Li Jun

doi:10.1007/BF02836471

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Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection

OriginalPaper | Updated：2021-08-27

- Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection
- Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection
- 中国结合医学杂志（英文版） 2005年11卷第2期页码：136-141
- Affiliations：
  
  1. General Hospital of Tianjin Medical University,Tianjin
  2. Nankai Hospital,China
- Author bio：
- Funds：
  
  Supported Financially by Tianjin Science and Technology Committee (No. 98113411)
- DOI：10.1007/BF02836471
  中图分类号：
- 纸质出版日期：2005，
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Yong-hao, Y., Nai-qiang, C., Qiang, F. et al. Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection., Chin. J. Integr. Med. 11, 136–141 (2005). https://doi.org/10.1007/BF02836471

Yu Yong-hao, Cui Nai-qiang, Fu Qiang, et al. Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection[J]. Chinese Journal of Integrative Medicine, 2005,11(2):136-141.
Yong-hao, Y., Nai-qiang, C., Qiang, F. et al. Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection., Chin. J. Integr. Med. 11, 136–141 (2005). https://doi.org/10.1007/BF02836471 DOI：

Yu Yong-hao, Cui Nai-qiang, Fu Qiang, et al. Change of Th1/Th2 cytokine equilibrium in rats with severe sepsis and therapeutic effect of recombinant interleukin-12 and Shenmai injection[J]. Chinese Journal of Integrative Medicine, 2005,11(2):136-141. DOI： 10.1007/BF02836471.

摘要

Objective: To evaluate the dynamic change of Thl/Th2 cytokines in serum

peritoneal lavage fluid (PLF) and splenic macrophages (SM) in rats with severe peritonitis

and to observe the therapeutic effects of recombinant interleukin-12 (rlL-2) and Shenmai injection (SMI)

a Chinese medicinal preparation.Methods: Severe peritonitis (SP) model was induced by intraperitoneal injection of E. coli and B. frag

and mild peritonitis (MP) model was induced by cecal ligation and punching. Then the following experiments were done: (1) Survival rates of animals after every 6 hrs in the 72 hrs after modeling were recorded

serum and PLF levels of cytokines

including tumor necrosis factor-α (TNF-α)

interferon-γ (IFN-γ)

and interleukin-10 (IL-10)

6 hrs

12 hrs

24 hrs and 48 hrs after modeling were measured. (2) Model rats were treated with rIL-12 or SMI

the survival rate was recorded and serum levels of TNF-α

INF-γ

and IL-10 before and after treatment were measured

and (3) amount of these cytokines produced by SM were determined 6 hrs

12 hrs and 24 hrs after treatment. The survival rates and levels of cytokines were then compared between the groups (model group treated with rIL-12 or SMI

untreated model group

and blank group).Results: Serum and PLF levels of IFN-γ

TNF-α at all the time points in SP rats were significantly lower than those in MP rats while those of IL-10 6 hrs and 12 hrs after modeling were significantly higher in the former than that in the latter (P<0.05). IFN-γ secretion of SM in SP rats was significantly higher than that in MP rats 6 hrs after modeling (P<0. 05). Administration of rlL-12 or SMI given before modeling could improve the survival rate of the model rats (P<0.05) and cause significant increase of the serum level and SM secretion of IFN-γ.Conclusion: Imbalance in promoting/antagonizing inflammatory cytokines and Th2 response dominance appear in SP rats early at the initiating stage

and SM secretion of inflammation promoting factor also reduces. Administration in time of rIL-12 and SMI

may increase the survival rate

and its mechanism may be related with their immuno-stimulating action.

Abstract

Objective: To evaluate the dynamic change of Thl/Th2 cytokines in serum

peritoneal lavage fluid (PLF) and splenic macrophages (SM) in rats with severe peritonitis

and to observe the therapeutic effects of recombinant interleukin-12 (rlL-2) and Shenmai injection (SMI)

a Chinese medicinal preparation.Methods: Severe peritonitis (SP) model was induced by intraperitoneal injection of E. coli and B. frag

serum and PLF levels of cytokines

including tumor necrosis factor-α (TNF-α)

interferon-γ (IFN-γ)

and interleukin-10 (IL-10)

6 hrs

12 hrs

24 hrs and 48 hrs after modeling were measured. (2) Model rats were treated with rIL-12 or SMI

the survival rate was recorded and serum levels of TNF-α

INF-γ

and IL-10 before and after treatment were measured

and (3) amount of these cytokines produced by SM were determined 6 hrs

12 hrs and 24 hrs after treatment. The survival rates and levels of cytokines were then compared between the groups (model group treated with rIL-12 or SMI

untreated model group

and blank group).Results: Serum and PLF levels of IFN-γ

and SM secretion of inflammation promoting factor also reduces. Administration in time of rIL-12 and SMI

may increase the survival rate

and its mechanism may be related with their immuno-stimulating action.

关键词

sepsisimmuno-modulatory cytokineinterleukintumor necrosis factorinterferonsplenic macrophageShenmai injectionrat

Keywords

sepsisimmuno-modulatory cytokineinterleukintumor necrosis factorinterferonsplenic macrophageShenmai injectionrat

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