A new rat model of cerebral infarction based on the injury of vascular endothelial cell

Chen Yi; Ge Jin-wen; Deng Bing-xiang

doi:10.1007/BF02836504

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A new rat model of cerebral infarction based on the injury of vascular endothelial cell

OriginalPaper | Updated：2021-08-27

- A new rat model of cerebral infarction based on the injury of vascular endothelial cell
- A new rat model of cerebral infarction based on the injury of vascular endothelial cell
- 中国结合医学杂志（英文版） 2005年11卷第3期页码：195-200
- Affiliations：
  
  1. Department of Physiology, Hunan University of Traditional Chinese Medicine,Changsha
  2. Department of Science and Technology, Hunan University of Traditional Chinese Medicine,Changsha
- Author bio：
- Funds：
  
  Supported by Young-middle Aged Science and Technological Fund of Hunan Province (No. 00JZY2145)
- DOI：10.1007/BF02836504
  中图分类号：
- 纸质出版日期：2005，
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Yi, C., Jin-wen, G. & Bing-xiang, D. A new rat model of cerebral infarction based on the injury of vascular endothelial cell., Chin. J. Integr. Med. 11, 195–200 (2005). https://doi.org/10.1007/BF02836504

Chen Yi, Ge Jin-wen, Deng Bing-xiang. A new rat model of cerebral infarction based on the injury of vascular endothelial cell[J]. Chinese Journal of Integrative Medicine, 2005,11(3):195-200.
Yi, C., Jin-wen, G. & Bing-xiang, D. A new rat model of cerebral infarction based on the injury of vascular endothelial cell., Chin. J. Integr. Med. 11, 195–200 (2005). https://doi.org/10.1007/BF02836504 DOI：

Chen Yi, Ge Jin-wen, Deng Bing-xiang. A new rat model of cerebral infarction based on the injury of vascular endothelial cell[J]. Chinese Journal of Integrative Medicine, 2005,11(3):195-200. DOI： 10.1007/BF02836504.

摘要

Objective: A new rat model of cerebral infarction was developed to elucidate the contribution of vascular endothelial cell during focal cerebral infarction formation.Methods: Forty-eight Sprague-Dawley (SD) rats were randomly divided into the model group

sham operation group

and control group for indexes observation of triphenyltetrazolium chloride (TTC) dyeing

neurological deficit

plasma tissue-type plasminogen activator (tPA) activity

plasminogen activator inhibitor (PAD activity

thromboxane B2(TXB2) content

and 6-keto-prostaglandin (6-keto-PGF1α) content.Results: (1) The highest neurological score appeared at 6 h after operation

descending significantly at sequential time. (2) Using TTC dyeing and optical microscope technique

pathological changes in brains were observed. (3) Compared with control group and sham operation groups

there was a decrease in tPA activity of model rats at the initial 12 h after injection of sodium laurate (P<0. 05)

PAI activity decreased markedly in the model group at 24 h after injection of sodium laurate. (4) In plasma TXB2 concentration reached the highest level compared at 6 h after injection of sodium laurate

but there were not obvious differences in plasma 6-keto-PGF1α

concentration among all groups (P> 0. 05).Conclusion: Focal cerebral infarction in rats could be induced by some sodium laurate

showing ischemic cerebrum necrosis

function disorder of vascular endothelium-platelet

fibrinolysis abnormality. This model could play an important role in researching the contribution of vascular endothelial cell during cerebral infarction development

preventing and curing by traditional Chinese medicine.

Abstract

sham operation group

and control group for indexes observation of triphenyltetrazolium chloride (TTC) dyeing

neurological deficit

plasma tissue-type plasminogen activator (tPA) activity

plasminogen activator inhibitor (PAD activity

thromboxane B2(TXB2) content

and 6-keto-prostaglandin (6-keto-PGF1α) content.Results: (1) The highest neurological score appeared at 6 h after operation

descending significantly at sequential time. (2) Using TTC dyeing and optical microscope technique

pathological changes in brains were observed. (3) Compared with control group and sham operation groups

there was a decrease in tPA activity of model rats at the initial 12 h after injection of sodium laurate (P<0. 05)

but there were not obvious differences in plasma 6-keto-PGF1α

concentration among all groups (P> 0. 05).Conclusion: Focal cerebral infarction in rats could be induced by some sodium laurate

showing ischemic cerebrum necrosis

function disorder of vascular endothelium-platelet

fibrinolysis abnormality. This model could play an important role in researching the contribution of vascular endothelial cell during cerebral infarction development

preventing and curing by traditional Chinese medicine.

关键词

sodium laurateCerebral InfarctionVascular Endothelial Cell

Keywords

sodium laurateCerebral InfarctionVascular Endothelial Cell

references

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