Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase

Chen Zhi-qiang; Huang Huai-peng; Huang Wen-zheng; Zhu Xiao-di; Lin Qing-qi

doi:10.1007/BF02838036

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Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase

OriginalPaper | Updated：2021-08-27

- Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase
- Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase
- 中国结合医学杂志（英文版） 2003年9卷第3期页码：210-214
- Affiliations：
  
  1. Institute of Integrative Chinese and Western Medicine, Hebei Medical University,Shijiazhuang
  2. Nanjing University of TCM,China
  3. The Affiliated Hospital of Tianjin College of TCM,China
- Author bio：
- Funds：
  
  The item was supported by Tianjin Scientific Committee (No. 953101311) and won the second grade Science and Technology Progress award of Tianjin in 2001
- DOI：10.1007/BF02838036
  中图分类号：
- 纸质出版日期：2003，
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Zhi-qiang, C., Huai-peng, H., Wen-zheng, H. et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase., Chin. J. Integr. Med. 9, 210–214 (2003). https://doi.org/10.1007/BF02838036

Chen Zhi-qiang, Huang Huai-peng, Huang Wen-zheng, et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase[J]. Chinese Journal of Integrative Medicine, 2003,9(3):210-214.
Zhi-qiang, C., Huai-peng, H., Wen-zheng, H. et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase., Chin. J. Integr. Med. 9, 210–214 (2003). https://doi.org/10.1007/BF02838036 DOI：

Chen Zhi-qiang, Huang Huai-peng, Huang Wen-zheng, et al. Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase[J]. Chinese Journal of Integrative Medicine, 2003,9(3):210-214. DOI： 10.1007/BF02838036.

摘要

Objective: To explore the effect of the Nephritis No. 3 (N-3) recipe on nitric oxide (NO)

nitric oxide synthase (NOS) secreted by cultured mesangial cells (MC) and its gene expression of the inducible nitric oxide synthase (iNOS).Methods; The drug (nephritis No. 3)-containing serum was prepared with serum pharmacological technique

and then was applied to react on mesangial cells cultured in fetal calf serum (FCS) and cells cultured in FCS plus lipopolysaccharide. To observe the secretion of NO and NOS and the gene expression of iNOS by means of RT-PCR.Results: Under the two kinds of culture conditions

the content of NO and NOS in the groups with drug-containing serum were higher than those without drug-containing serum (P < 0.05

P < 0.01)

and the expression of iNOS mRNA was up-regulated too.Conclusion: The N-3 could significantly promote the secretion of NO and NOS and the mRNA expression of iNOS in rats.

Abstract

Objective: To explore the effect of the Nephritis No. 3 (N-3) recipe on nitric oxide (NO)

the content of NO and NOS in the groups with drug-containing serum were higher than those without drug-containing serum (P < 0.05

P < 0.01)

and the expression of iNOS mRNA was up-regulated too.Conclusion: The N-3 could significantly promote the secretion of NO and NOS and the mRNA expression of iNOS in rats.

关键词

Nephritis No. 3 recipemesangial cellNitric Oxidenitric oxide synthase

Keywords

Nephritis No. 3 recipemesangial cellNitric Oxidenitric oxide synthase

references

WANG DH, XU XM. NO and nephrosclerosis. Foreign Med, Urolog Fascicles 1998; 18(1): 6–8.

LI XM, DAI ZH, WANG HY. Glomerular MC hyperplastic inhibitory factor. Foreign Med, Urolog Fascicles 1997; 17(1): 31–33.

JIANG DX, HUANG WZ, YANG HT, et al. Experimental study of N-3 recipe on glomerular MC proliferation and its generation of TNF-α. Chin J Basic Med 1999; 5(10): 36–39.

CHEN YP, GAO J, XING BC, et al. Culture of glomerular MC. Acad J Beijing Med Univ 1989; 21(4): 335 -336.

HU MC, YU BS. Action of NO in nephropathy. Chin J Nephropathy 1996; 12 (2): 121–123.

CHEN M, HUANG SM. Enthothelin, NO and diabetic nephropathy. Foreign Med, Endocrinology Fascicle 1999; 19(2): 69–72.

Trachtman H, Futteweit S, Singhal PC. Nitric oxide modulates the synthesis of extracellular matrix protein in cultured rat mesangeal cells. Biochem Biophys Res Commun 1995; 270(1): 120–125.

Trachtman H, Futteweit S, Grag P, et al. Nitric oxide stimulates the activity of a 72-Kda neutral matrix metalloproteinase in cultured rat mesangeal cells. Biochem Biophys Res Commun 1996; 218(3): 704–708.

ZHONG CS, SUN AY. Biomedicine of nitric oxide. Shanghai! Shanghai Med Univ Press, 1997: 256–257.

CHEN ZQ, HUANG HP, HUANG WZ, et al. Effect of N-3 recipe on TGF-α 1 of glomerular MC and its mRNA expression. Chin J TCM Basic Med 2002; 8(9): 46 -48.

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