Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α

Ge Jin-wen; Chen Song; Zhu Hui-bin

doi:10.1007/BF02838039

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Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α

OriginalPaper | Updated：2021-08-27

- Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α
- Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α
- 中国结合医学杂志（英文版） 2003年9卷第3期页码：219-223
- Affiliations：
  
  Laboratory of Vascular Biology, Hunan College of TCM,Changsha,China
- Author bio：
- Funds：
  
  The item is supported by Natural Science Foundation of Hunan Province (No. 98JJY 2027) and Young-middle Aged Science and Technological Fund of Hunan Province (NO. 00JZYZ145)
- DOI：10.1007/BF02838039
  中图分类号：
- 纸质出版日期：2003，
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Jin-wen, G., Song, C. & Hui-bin, Z. Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α., Chin. J. Integr. Med. 9, 219–223 (2003). https://doi.org/10.1007/BF02838039

Ge Jin-wen, Chen Song, Zhu Hui-bin. Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α[J]. Chinese Journal of Integrative Medicine, 2003,9(3):219-223.
Jin-wen, G., Song, C. & Hui-bin, Z. Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α., Chin. J. Integr. Med. 9, 219–223 (2003). https://doi.org/10.1007/BF02838039 DOI：

Ge Jin-wen, Chen Song, Zhu Hui-bin. Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α[J]. Chinese Journal of Integrative Medicine, 2003,9(3):219-223. DOI： 10.1007/BF02838039.

摘要

Objective: To observe the changes of coagulation and fibrinolysis function in human umbilical cord vein endothelial cells (HUVEC) induced by recombinant human tumor necrosis factor-α (rhTNF-α) and the effect of Naotaifang extract (NTE) on it.Methods: Cultured HUVEC is randomly divided into six groups: Control group

NTE control group (only 2 g/L NTE)

rhTNF-α group (100 μg/ L rhTNF-α)

and low-dosage

middle-dosage and high-dosage NTE group (100 μg/L rhTNF-α and 0. 67 g/L

2 g/L

6 g/L NTE). The coagulation activity of frozen-dissolved HUVEC

von Willebrand factor (vWF) content in the conditioned medium and tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAD activity were to be detected after 24 hrs.Results: Compared with the control group

PAI activity were enhanced

vWF release markedly increased in conditioned medium of TNF-α group (P < 0.01) and the frozen-dissolved HUVEC markedly shortens the rabbit plasma prothrombin time

and the above changes could be significantly inhibited by the 3 dosages of NTE (P < 0.05

P < 0. 01).Conclusion: NTE is effective in inhibiting the coagulation activity of the HUVEC non-stimulated or stimulated by rhTNF-α to enhance the vWF release

and to adjust fibrinolytic function

and mainly to inhibit the PAI activity.

Abstract

NTE control group (only 2 g/L NTE)

rhTNF-α group (100 μg/ L rhTNF-α)

and low-dosage

middle-dosage and high-dosage NTE group (100 μg/L rhTNF-α and 0. 67 g/L

2 g/L

6 g/L NTE). The coagulation activity of frozen-dissolved HUVEC

PAI activity were enhanced

vWF release markedly increased in conditioned medium of TNF-α group (P < 0.01) and the frozen-dissolved HUVEC markedly shortens the rabbit plasma prothrombin time

and the above changes could be significantly inhibited by the 3 dosages of NTE (P < 0.05

P < 0. 01).Conclusion: NTE is effective in inhibiting the coagulation activity of the HUVEC non-stimulated or stimulated by rhTNF-α to enhance the vWF release

and to adjust fibrinolytic function

and mainly to inhibit the PAI activity.

关键词

Naotaifang extracttumor necrosis factorVascular Endothelial Cell

Keywords

Naotaifang extracttumor necrosis factorVascular Endothelial Cell

references

HE YH, HAO XY, GE JW, et al. Clinical study of Naotaifang in treating Qixu Xueyu type cerebral infarction. Chin TCM Emergency 2001; 10(6): 319–321.

HE YH, GE JW, CHENG ZY, et al. Effect of Naotaifang on TXB2, 6-keto-PGF1α and serum TNF-α content in patients with cerebral infarction. Chin J TCM Inform 2002; 9(4): 16–17.

CHEN S, GE JW, DENG BX. Study of Naotaifang extract on anti-TNFα induced human HUVEC injury. Acad J Hunan College of TCM 2002; 22(4): 7–8.

Jaffe EA, Nachman RL, Becker CG, et al. Culture of human endothelial cells derived from umbilical veins. J Clin Invest 1973; 52(11): 2745–2756.

LI ZG, WEN ZB, HAN JZ, et al. Relationship of thrombin on effect of bovine aortic endothelial tissue factor activity and PKC transmission pathway. Chin J Hematol 1999; 20(3): 130–133.

Bombeli T, Karsan A, Tait JF, et al. Apoptotic vascular endothelial cells become procoagulant. Blood 1997; 89 (7): 2429–2442.

SHANG GP, WEN ZB, LI JC, et al. Effect of Buyang Huanwu decoction on thrombin induced VEC released NO, vWF, TFPI and expressed tissue factor. Acad J Hunan Med Univ 2000; 27(4): 315–318.

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