Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis

Xie Qiang-min; Chen Ying; Wu Xi-mei; Chen Ji-qiang; Bian Ru-lian

doi:10.1007/BF02838568

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Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis

OriginalPaper | Updated：2021-08-27

- Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis
- Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis
- 中国结合医学杂志（英文版） 2003年9卷第2期页码：128-131
- Affiliations：
  
  Key Laboratory of SDA, Medical College of Zhejiang University,Hangzhou
- Author bio：
- Funds：
  
  This program was supported by the National New Drugs Foundation (No.98-35-N-13)
- DOI：10.1007/BF02838568
  中图分类号：
- 纸质出版日期：2003，
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Qiang-min, X., Ying, C., Xi-mei, W. et al. Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis., Chin. J. Integr. Med. 9, 128–131 (2003). https://doi.org/10.1007/BF02838568

Xie Qiang-min, Chen Ying, Wu Xi-mei, et al. Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis[J]. Chinese Journal of Integrative Medicine, 2003,9(2):128-131.
Qiang-min, X., Ying, C., Xi-mei, W. et al. Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis., Chin. J. Integr. Med. 9, 128–131 (2003). https://doi.org/10.1007/BF02838568 DOI：

Xie Qiang-min, Chen Ying, Wu Xi-mei, et al. Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis[J]. Chinese Journal of Integrative Medicine, 2003,9(2):128-131. DOI： 10.1007/BF02838568.

摘要

Objective: To study the therapeutic mechanism of Santeng Dingtong recipe (STDT) on monosodium urate crystal (MSU) induced rabbit arthritis.Methods: Forty-two rabbits were randomly divided into six groups

7 in each group. Group 1 received 0.9% saline 2.5 ml/kg per day by gastrogavage (ig) for 10 days; Group 2

3 and 4 received STDT 0.125 g/kg

1.0 g/kg and 8.0 g/kg per day respectively by ig for 10 days; Group 5 received colchicine 4.5 mg/kg per day by ig for 4 days; and Group 6 was untreated. MSU crystals 10 mg /500 μl containing polymyxin B 10 u/ml was injected into the knee joints of Group 1–5 to make rabbit arthritis models. Leukocytes in synovial lavage fluids was then counted and differentiated; pathological injury of synovial membranes was observed under HE staining; interleukin-1 beta (IL-lβ)

tumor necrosis factor alpha (TNFα) and leukotriene B4 (LTB4) content in synovial lavage fluids were determined by ELISA.Results: MSU caused a rapid leukocyte infiltration and increased production of IL-lβ

TNFα and LTB4 2 hrs after intra-articular injection. STDT inhibited neutrophil infiltration in synovial fluids dose-dependently

protected the synovial membrane against pathological injury and reduced the production of IL-lβ

TNFα and LTB4; while colchicine did not decrease the level of TNFα

but significantly inhibited neutrophil infiltration in synovial fluid and reduced the production of IL-11β and LTB4.Conclusion: STDT exerts an anti-inflammatory effect in rabbit model of acute MSU arthritis

its mechanism being probably due to the decrease of IL-1β

TNFα and LTB4 synthesis.

Abstract

7 in each group. Group 1 received 0.9% saline 2.5 ml/kg per day by gastrogavage (ig) for 10 days; Group 2

3 and 4 received STDT 0.125 g/kg

TNFα and LTB4 2 hrs after intra-articular injection. STDT inhibited neutrophil infiltration in synovial fluids dose-dependently

protected the synovial membrane against pathological injury and reduced the production of IL-lβ

TNFα and LTB4; while colchicine did not decrease the level of TNFα

its mechanism being probably due to the decrease of IL-1β

TNFα and LTB4 synthesis.

关键词

Santeng Dingtong recipecolchicinemonosodium urate crystalrabbitArthritisinterleukin-1 betatumor necrosis factor alphaleukotriene B4enzyme linked immunosorbent assay

Keywords

Santeng Dingtong recipecolchicinemonosodium urate crystalrabbitArthritisinterleukin-1 betatumor necrosis factor alphaleukotriene B4enzyme linked immunosorbent assay

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State Pharmacopoeia Committee. Pharmacopoeia of People’s Republic of China. 2000; part I : 543.

Matsukawa A, Yoshimura T, Maeda T, et al. Analysis of the cytokine network among tumor necrosis factor alpha, interleukin-1beta, interleukin-8, and interleukin-1 receptor antagonist in monosodium urate crystal-induced rabbit arthritis. Lab Invest 1998; 78(5): 559–569.

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