Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice

Zhou Lin-fu; Yin Kai-sheng; Zhou Zhi-min

doi:10.1007/BF02838615

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Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice

OriginalPaper | Updated：2021-08-27

- Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice
- Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice
- 中国结合医学杂志（英文版） 2003年9卷第4期页码：281-284
- Affiliations：
  
  Department of Respiratory Diseases, the First Affiliated Hospital of Nanjing Medical University,Nanjing
- Author bio：
- Funds：
  
  This study was supported by a research foundation from Jiangsu Provincial Administration Bureau of TCM (No. 9974)
- DOI：10.1007/BF02838615
  中图分类号：
- 纸质出版日期：2003，
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Lin-fu, Z., Kai-sheng, Y. & Zhi-min, Z. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice., Chin. J. Integr. Med. 9, 281–284 (2003). https://doi.org/10.1007/BF02838615

Zhou Lin-fu, Yin Kai-sheng, Zhou Zhi-min. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice[J]. Chinese Journal of Integrative Medicine, 2003,9(4):281-284.
Lin-fu, Z., Kai-sheng, Y. & Zhi-min, Z. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice., Chin. J. Integr. Med. 9, 281–284 (2003). https://doi.org/10.1007/BF02838615 DOI：

Zhou Lin-fu, Yin Kai-sheng, Zhou Zhi-min. Role of low dosage arsenic trioxide on pulmonary dendritic cells in asthmatic mice[J]. Chinese Journal of Integrative Medicine, 2003,9(4):281-284. DOI： 10.1007/BF02838615.

摘要

Objective: To investigate the distribution and recruitment of pulmonary dendritic cells (DCs) and the influence of low dosage arsenic trioxide (As2O3) on them in the airway of asthmatic mice.Methods: Thirty BALB/c mice were randomly divided into 3 groups: the control group

the asthmatic group and the As2 O3 treated group. The mice asthmatic model was induced via sensitizing with peritoneal injection of ovalbumin (OVA) for two times and then provocated with aerosol inhalation of OVA for a week. The treated group was peritoneally injected with 0. 2 ml solution of As2O3 (4mg/kg) 0. 5h after each provocation. The immunohistochemistry and computerised image analysis were applied to detect quantitatively the DCs in the lung and airway of mice.Results: All intraepithelial nonlymphoid dendritic cells-145 (NLDC-145) throughout the respiratory tree in the mice of the control group formed a network with the density of DCs varying from (575±54) cells/mm2 epithelial surface in the large airway

to (68±12) cells/ mm2 epithelial surface in the small airway. The distribution of airway NLDC-145+ in the asthmatic group was similar to that in the control group

but its density was significantly upregulated (P < 0.01). The distribution of airway NLDC-145 in the treated group was similar to that in the asthmatic group

only its density was significantly downregulated (P < 0.01).Conclusion: There is an integral network of NLDC-145+ throughout the respiratory tree. To downregulate the density but not change the distribution of pulmonary DCs could be an important therapeutic mechanism of low dosage As2 O3 in treating asthma.

Abstract

to (68±12) cells/ mm2 epithelial surface in the small airway. The distribution of airway NLDC-145+ in the asthmatic group was similar to that in the control group

but its density was significantly upregulated (P < 0.01). The distribution of airway NLDC-145 in the treated group was similar to that in the asthmatic group

关键词

Arsenic Trioxidebronchial asthmadendritic cellimmunohistochemistry

Keywords

Arsenic Trioxidebronchial asthmadendritic cellimmunohistochemistry

references

Mervis J. Ancient remedy performs new tricks. Science 1996; 273 (5275): 578.

YIN KS, YAO X, CHEN JD, et al. Primary study on the effect of arsenic trioxide on eosinophils in the conducting airways of asthmatic guinea-pigs. Acta Nanjing Med Univ 1999; 19 (5): 433.

Power CA, Church DJ, Meyer A, et al. Cloning and characterization of a specific receptor for the novel CC chemochine MIP-3α from lung dendritic cells. J Exp Med 1997; 186 (6): 825–835.

Lambert LE, Berling JS, Kudlacz EM. Charaterization of the antigen-presenting cell and T cell requirements for induction of pulmonary eosinophilia in a murine model of asthma. Clin Immunol Immunopathol 1996; 81 (3): 307–311.

Inaba K, Swiggard WJ, Inaba M, et al. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. Cell Immunol 1995; 163 (1): 148–156.

Nelson DJ, McWilliam AS, Haining S, et al. Modulation of airway intraepithelial dendritic cells following exposure to steroids. Am J Respir Crit Care Med 1995; 151 (2): 475–481.

Brokaw JJ, White GW, Baluk P, et al. Glucocorticoidsinduced apoptosis of dendritic cells in the rat tracheal mucosa. Am J Respir Cell Mol Biol 1998; 19 (4): 598–605.

Hansen G, McIntireJJ, Yeung VP, et al. CD4+T helper cells engineered to produce latent TGF-β1 reverse allergen-induced airway hyper-reactivity and inflammation. J Clin Invest 2000; 105 (1): 61–70.

Pardigen N, Bercovici N, Calbo S, et al. Roles of co-stimulation in CD8+ T cell activation. Int Immunol 1998; 10 (5): 619–630.

Dai J, Weinberg RS, Waxman S, et al. Malignant cells can be sensitized to undergo growth inhibition and apoptosis by arsenic trioxide through modulation of the glutathione redox system. Blood 1999; 93 (1): 268–277.

CHEN GQ, SHI XG, TANG W, et al. Use of arsenic trioxide (AS2O3) in the treatment of acute promyelocytic leukemia (APL): I. As2O3 exerts dose-dependent dual effects on APL cells. Blood 1997; 89 (9): 3345–3353.

LI Y, YAO H, SUN ML, et al. Studies of the developmental toxicity of arsenic on culture embryo in vitro. Chin J Endemiol 1996; 15 (2): 93–96.

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