Effect of Reduqing ( ) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood

Wun Tzechein; Li Anguo; Li Mingzhen; Lu Fuer

doi:10.1007/BF02935050

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Effect of Reduqing ( ) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood

OriginalPaper | Updated：2021-08-27

- Effect of Reduqing ( ) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood
- Effect of Reduqing ( ) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood
- 中国结合医学杂志（英文版） 1998年4卷第1期页码：43-47
- Affiliations：
  
  1. Searle-Monsanto Co.,USA
  2. Institution of Integration of Traditional Chinese and Western Medicine, Tongji Medical University,Wuhan,P. R. China
- Author bio：
- Funds：
- DOI：10.1007/BF02935050
  中图分类号：
- 纸质出版日期：1998，
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Tzechein, W., Anguo, L., Mingzhen, L. et al. Effect of Reduqing () on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood., CJIM 4, 43–47 (1998). https://doi.org/10.1007/BF02935050

Wun Tzechein, Li Anguo, Li Mingzhen, et al. Effect of Reduqing ( ) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood[J]. Chinese Journal of Integrative Medicine, 1998,4(1):43-47.
Tzechein, W., Anguo, L., Mingzhen, L. et al. Effect of Reduqing () on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood., CJIM 4, 43–47 (1998). https://doi.org/10.1007/BF02935050 DOI：

Wun Tzechein, Li Anguo, Li Mingzhen, et al. Effect of Reduqing ( ) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood) on endotoxin-lnduced production of tissue factor and cytokines in human whole blood[J]. Chinese Journal of Integrative Medicine, 1998,4(1):43-47. DOI： 10.1007/BF02935050.

摘要

Objective: To study the effect of Reduqing (RDQ) Injection on the lipopolysaccharide (LPS)-induced tissue factor and cytokine production in whole blood. Methods: Heparinized human blood was incubated with LPS in the presence or absence of RDQ. The plasma concentrations of TNF-α

IL-1β

IL-6

and IL-8 were measured by enzyme-linked immunosorbent assays (ELISA) and the monocyte tissue factor activity was measured by a one-stage tissue factor induced plasma clotting time assay. Results: RDQ was found to diminish the LPS-induced increase of TNF-α

IL-1β and IL-6 in plasma but did not completely abolish their production. In contrast to the effect on these cytokines

RDQ caused further increase of the plasma level of IL-8 and the monocyte TF activity in the presence of LPS. Conclusions: In the in vitro whole blood assay system used in this study

the decrease of LPS-induced production of TNF-α

IL-1β

and IL-6 was similar to a previousin vivo study on the effect of RDQ on the production of these cytokines in response to two-time LPS injection in rabbits

while the increase of IL-8 and TF production was contradictory to the previous in vivo study. Potential reasons contributing to the differences are discussed.

Abstract

IL-1β

IL-6

IL-1β and IL-6 in plasma but did not completely abolish their production. In contrast to the effect on these cytokines

RDQ caused further increase of the plasma level of IL-8 and the monocyte TF activity in the presence of LPS. Conclusions: In the in vitro whole blood assay system used in this study

the decrease of LPS-induced production of TNF-α

IL-1β

and IL-6 was similar to a previousin vivo study on the effect of RDQ on the production of these cytokines in response to two-time LPS injection in rabbits

while the increase of IL-8 and TF production was contradictory to the previous in vivo study. Potential reasons contributing to the differences are discussed.

关键词

Reduqingtissue factortumor necrosis factor-αinterleukin-lβinterleukin-6interleukin-8lipopolysaccharidemonocytesmononuclear cells

Keywords

Reduqingtissue factortumor necrosis factor-αinterleukin-lβinterleukin-6interleukin-8lipopolysaccharidemonocytesmononuclear cells

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