Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

Wang Gang; Liu  Chun-tao; Wang Zeng-li; Yan Cun-liang; Luo Feng-ming; Wang Lei; Li Ting-qian

doi:10.1007/s11655-006-0262-y

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Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma

OriginalPaper | Updated：2021-08-27

- Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma
- Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma
- 中国结合医学杂志（英文版） 2006年12卷第4期页码：262-267
- Affiliations：
  
  1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University,Chengdu,China
  2. Department of Respiratory Disease, West China Hospital, Sichuan University,China
  3. Department of Laboratory Medicine, Shenzhen Hospital Affiliated to Peking University,China
- Author bio：
- Funds：
  
  Supported by Science Foundation of State Administration of Traditional Chinese Medicine (No. 04-05JQ03), and partly supported by China Postdoctoral Science Foundation (No. 2005037800).
- DOI：10.1007/s11655-006-0262-y
  中图分类号：
- 纸质出版日期：2006，
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Wang, G., Liu, Ct., Wang, Zl. et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma., Chin. J Integ. Med. 12, 262–267 (2006). https://doi.org/10.1007/s11655-006-0262-y

Wang Gang, Liu Chun-tao, Wang Zeng-li, et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma[J]. Chinese Journal of Integrative Medicine, 2006,12(4):262-267.
Wang, G., Liu, Ct., Wang, Zl. et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma., Chin. J Integ. Med. 12, 262–267 (2006). https://doi.org/10.1007/s11655-006-0262-y DOI：

Wang Gang, Liu Chun-tao, Wang Zeng-li, et al. Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma[J]. Chinese Journal of Integrative Medicine, 2006,12(4):262-267. DOI： 10.1007/s11655-006-0262-y.

摘要

To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Th1) specific transcription factor T-box expressed in T cells (T-bet) expression and Th1/Th2 equilibrium. The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon γ (IFN-γ) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4+CCR3+ and CD4+CCR5+ cells were counted by flow cytometry. The expression of T-bet mRNA and the level of IFN-γ were lower

but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 μ g/ml) intervention

the former two parameters raised and showed a positive correlation between them

while the level of IL-4 was decreased. The mean percentage of CD4+CCR3+ cells in asthma patients was significantly higher but that of CD4+CCR5+ cells was lower when compared with those in healthy subjects respectively. After AM intervention

the abnormal change in the two indexes was improved to certain extent

showing a reversing status of Th2 polarization. AM could increase the expression of T-bet mRNA and Th1 cytokines such as IFN-γ

and might reverse the Th2 predominant status in asthma patients.

Abstract

but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 μ g/ml) intervention

the former two parameters raised and showed a positive correlation between them

the abnormal change in the two indexes was improved to certain extent

showing a reversing status of Th2 polarization. AM could increase the expression of T-bet mRNA and Th1 cytokines such as IFN-γ

and might reverse the Th2 predominant status in asthma patients.

关键词

AsthmaAstragalus membranaceustranscription factorT-box expressed in T cellsT helper cellsTinterferon-γ

Keywords

AsthmaAstragalus membranaceustranscription factorT-box expressed in T cellsT helper cellsTinterferon-γ

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