Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress

Zhang Xiao; Dong Yi-long; Yang Nan; Liu Yan-yong; Gao Rui-feng; Zuo  Ping-ping

doi:10.1007/s11655-007-0046-4

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Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress

OriginalPaper | Updated：2021-08-27

- Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress
- Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress
- 中国结合医学杂志（英文版） 2007年13卷第1期页码：46-49
- Affiliations：
  
  Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973) (NoG2000057000)
- DOI：10.1007/s11655-007-0046-4
  中图分类号：
- 纸质出版日期：2007，
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Zhang, X., Dong, Yl., Yang, N. et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress., Chin. J Integ. Med. 13, 46–49 (2007). https://doi.org/10.1007/s11655-007-0046-4

Zhang Xiao, Dong Yi-long, Yang Nan, et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress[J]. Chinese Journal of Integrative Medicine, 2007,13(1):46-49.
Zhang, X., Dong, Yl., Yang, N. et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress., Chin. J Integ. Med. 13, 46–49 (2007). https://doi.org/10.1007/s11655-007-0046-4 DOI：

Zhang Xiao, Dong Yi-long, Yang Nan, et al. Effects of Ning Shen Ling Granule (宁神灵冲剂) and dehydroepiandrosterone on cognitive function in mice undergoing chronic mild stress[J]. Chinese Journal of Integrative Medicine, 2007,13(1):46-49. DOI： 10.1007/s11655-007-0046-4.

摘要

To investigate the changes of spontaneous and cognitive behavior

and cholinergic M receptors in the brain of mice subjected to chronic mild stress (CMS)

and to determine the effect of Ning Shen Ling Granule (宁神灵冲剂

NSL) and dehydroepiandrosterone (DHEA) on them. CMS model mice were established by applying stress every day for 3 consecutive weeks with 7 kinds of unforeseeable stress sources

and they were medicated for 1 week beginning at the 3rd week of modeling. The changes in behavior were determined by Morris Water Maze and spontaneous movement test

and M-receptor binding activity in cerebral cortex

hippocampus and hypothalamus were measured by radioactive ligand assay with 3H-QNB. (1) The spontaneous movement in CMS model mice was significantly reduced

with the latency for searching platform in Morris Water Maze obviously prolonged (P<0.01)

and these abnormal changes in behavior were improved in those treated with NSL and DHEA. (2) The binding ability of M-receptor in cerebral cortex and hippocampus of CMS mice was significantly decreased as compared with those in the control group (P<0.05)

but could be restored to the normal level after intervention with NSL or DHEA. The decline of spontaneous movement and spatial learning and memory ability could be induced in animals by chronic mild stress

and that may be related to the low activity of central cholinergic M-receptors. Both NSL and DHEA could effectively alleviate the above-mentioned changes.

Abstract

To investigate the changes of spontaneous and cognitive behavior

and cholinergic M receptors in the brain of mice subjected to chronic mild stress (CMS)

and to determine the effect of Ning Shen Ling Granule (宁神灵冲剂

NSL) and dehydroepiandrosterone (DHEA) on them. CMS model mice were established by applying stress every day for 3 consecutive weeks with 7 kinds of unforeseeable stress sources

and they were medicated for 1 week beginning at the 3rd week of modeling. The changes in behavior were determined by Morris Water Maze and spontaneous movement test

and M-receptor binding activity in cerebral cortex

hippocampus and hypothalamus were measured by radioactive ligand assay with 3H-QNB. (1) The spontaneous movement in CMS model mice was significantly reduced

with the latency for searching platform in Morris Water Maze obviously prolonged (P<0.01)

and that may be related to the low activity of central cholinergic M-receptors. Both NSL and DHEA could effectively alleviate the above-mentioned changes.

关键词

chronic mild stressed modelNing Shen Ling Granuledehydroepiandrosteronecentral cholinergic M-receptorspontaneous movementMorris water maze

Keywords

chronic mild stressed modelNing Shen Ling Granuledehydroepiandrosteronecentral cholinergic M-receptorspontaneous movementMorris water maze

references

Kang DW, ZR, Zhu WL, et al. Effects of Chaihujialonggumuli decoction on activity of hypothalamic-pituitary-adrenal axis in depression model. Chin J Clin Pharmacol Ther 2005;10(11): 1231–1235.

Zhang YZ, Nie HM, Hu YL, et al. Behavior study on Chaihu plus Longgu Muli Decoction treat in melancholia mouse model. Chin J Basic Med Tradit Chin Med 2003; 7(7): 30–32.

He YM, Du SL. Advancement in research on antidepressant of TCM. Hunan Guiding J Tradit Chin Med Pharmacol 2003; 9(6): 71–73.

Kroboth PD, Salek FS, Pittenger AL, et al. DHEA and DHEA-S: a review. J Clin Pharmaco 1999; 39: 327–348.

Bao L, Yang N, Liu YY, et al. Effects of dehydroepianddrosterone on cognitive function in mice with chronic mild stress. Chin J Clin Pharmacol Ther 2004; 9(2): 153–156.

Morris R. Developments of a water maze procedure for studying spatial learning in the rat. J Neurosci Methods 1984; 11: 47–60.

Willner P, Muscat R, Papp M. Chronic mild stress-induced anhedonia: a realistic animal model of depression. Neurosci Biobehav Rev 1992;16: 525–534.

Miller GE, Cohen S, Herbert TB. Pathways linking major depression and immunity in ambulatory female patients. Psychosomatic Med 1999; 61: 850–860.

Bloch M, Schmidt PJ, Danaceeau MA, et al. Dehydroepiandrosterone treatment of midlife dysthymia. Biol Psychiatry 1999; 45: 1533–1541.

Murphy BE. Steroids and depression. J Steriod Biochem Mol Bio 1991; 38: 537–59.

Baulieu EE, Thomas G, Legrain S, et al. Dehydroepiandrosterone (DHEA), DHEA sulfate, and aging: contribution of the DHEA Study to a socio-biomedical issue. Proc Natl Acad Sci 2000;97: 4279–4284.

Majewska MD. Neurosteroids: endogenous bimodal modulators of GABA-A receptor. Mechanism of action and physiological significance. Prog Neurobiol 1992;38: 379–395.

Chen, W. Chaihu plus Longgu Muli Decoction in treatment of depressive neurosis. Shaanxi Chin Med 1984;12: 41–43.

Ding SM. Guizhi Gancao Longgu Decoction in patients with neurosis: a report of 38 cases. Hubei J Chin Med 1983;1: 11–13.

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