Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro

Yan Lei; Qian Gao; Ke-ji Chen

doi:10.1007/s11655-008-0037-0

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Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro

OriginalPaper | Updated：2021-08-27

- Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro
- Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro
- 中国结合医学杂志（英文版） 2008年14卷第1期页码：37-41
- Affiliations：
  
  1. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Shijia Research Center, Peking University,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. A30070956)
- DOI：10.1007/s11655-008-0037-0
  中图分类号：
- 纸质出版日期：2008，
  
  网络出版日期：2008-6-21，
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Lei, Y., Gao, Q. & Chen, Kj. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro, ., Chin. J. Integr. Med. 14, 37–41 (2008). https://doi.org/10.1007/s11655-008-0037-0

Yan Lei, Qian Gao, Ke-ji Chen. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro[J]. Chinese Journal of Integrative Medicine, 2008,14(1):37-41.
Lei, Y., Gao, Q. & Chen, Kj. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro, ., Chin. J. Integr. Med. 14, 37–41 (2008). https://doi.org/10.1007/s11655-008-0037-0 DOI：

Yan Lei, Qian Gao, Ke-ji Chen. Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro[J]. Chinese Journal of Integrative Medicine, 2008,14(1):37-41. DOI： 10.1007/s11655-008-0037-0.

摘要

To study the effects of extracts from Panax notoginseng (EPN) and Panax ginseng fruit (EPGF) on the proliferation and migration of human umbilical vein endothelial cells (HUVECs) in vitro. Cell proliferation was determined using an MTT method with a cultured HUVECs model cell cycle analyzed by cytometry. The effect on endothelial cell migration was investigated using an agarose scraping method. The content of vascular endothelial growth factor (VEGF) in the supernate was determined by enzyme-linked immunosorbent assay (ELISA). The VEGF mRNA expression of vascular endothelial cells (VECs) with different concentrations of EPN and EPGF was examined by reverse transcriptase-polymerase chain reaction (RT-PCR). EPN and EPGF can promote the proliferation of VECs and the secretion of VEGF from HUVECs. It can increase the cell population significantly in the S phase to (15.22±1.33) % in the 50 mg/L dose group (P<0.05 or P<0.01). They can promote the VEC migration in the 200 mg/L dose group and the migration rate was 93.75% (P<0.01). They could also increase VEGF mRNA expression in VEC and the effects in the 100 mg/L and 50 mg/L dose groups were signifi cant with the proportion of VEGF mRNA expression of 0.1812±0.0413 and 0.2037±0.0399 respectively (P<0.01). EPN and EPGF can promote VEC proliferation

migration

DNA synthesis and VEGF mRNA expression. The results suggest that they have a certain effect on the genesis and development of new vessels in the ischemic myocardium.

Abstract

migration

DNA synthesis and VEGF mRNA expression. The results suggest that they have a certain effect on the genesis and development of new vessels in the ischemic myocardium.

关键词

endothelial cellvascular endothelial growth factorproliferationmigration

Keywords

endothelial cellvascular endothelial growth factorproliferationmigration

references

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Trochon V, Mabilat C, Bertrand P, et al. Evidence of involvement of CD44 in endothelial cell proliferation, migration and angiogenesis in vitro. Intl J Cancer 1996;66:664–668.

Lazarous DF, Shou M, Stiber JA, et al. Adenoviralmediated gene transfer induces sustained pericardial VEGF expression in dogs: effect on myocardial angiogenesis. Cardiovasc Res 1999;44:294–302.

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Dai RH, Li Y. Ischemic blood vessels producing in coronary heart disease and traditional Chinese medicine. Chin J Integr Tradit Chin West Med (Chin) 2002;20(3):163–164.

Li YJ, Deng HW, Chen X. Protective effect of ginsenosides and its ingredients on myocardial hypoxia, re-oxygen and ischemia-reperfusion injury. Acta Pharm Sin (Chin) 1987;22(1):1–5.

Li X, Chen JX, Sun JJ. Protective effect of panax notoginsenosides against myocardial ischemia-reperfusion injury in rats. Acta Pharmacol Sin (Chin) 1990;11(1):26–29.

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