Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate

Li-bin Zhan; Hua Sui; Xiao-guang Lu; Chang-kai Sun; Jian Zhang; Hui Ma

doi:10.1007/s11655-008-0117-1

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Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate

OriginalPaper | Updated：2021-08-27

- Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate
- Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate
- 中国结合医学杂志（英文版） 2008年14卷第2期页码：117-122
- Affiliations：
  
  1. The Second Affiliated Hospital, Dalian Medical University,Dalian,China
  2. Department of Pathophysiology, Dalian Medical University,Dalian,China
  3. Department of Emergency Medicine, Zhongshan Hospital, Dalian University,Dalian,China
  4. Department of Physiology, Institute for Brain Disorder, Dalian Medical University,Dalian,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30472255)
- DOI：10.1007/s11655-008-0117-1
  中图分类号：
- 纸质出版日期：2008，
  
  网络出版日期：2008-8-6，
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Zhan, Lb., Sui, H., Lu, Xg. et al. Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate., Chin. J. Integr. Med. 14, 117–122 (2008). https://doi.org/10.1007/s11655-008-0117-1

Li-bin Zhan, Hua Sui, Xiao-guang Lu, et al. Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate[J]. Chinese Journal of Integrative Medicine, 2008,14(2):117-122.
Zhan, Lb., Sui, H., Lu, Xg. et al. Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate., Chin. J. Integr. Med. 14, 117–122 (2008). https://doi.org/10.1007/s11655-008-0117-1 DOI：

Li-bin Zhan, Hua Sui, Xiao-guang Lu, et al. Effects of Zibu Piyin Recipe (滋补脾阴方药) on SNK-SPAR pathway in neuron injury induced by glutamate[J]. Chinese Journal of Integrative Medicine, 2008,14(2):117-122. DOI： 10.1007/s11655-008-0117-1.

摘要

To investigate the relationship between the excitotoxicity and serum-inducible kinase (SNK) and spine-associated Rap GTPase-activating protein (SPAR) pathway in primary hippocampal neuron injury induced by glutamate and furthermore

to explore the molecular mechanism of neuroprotection of Zibu Piyin Recipe (滋补脾阴方药

ZBPYR) and the relationship between ZBPYR and the morphological regulation of dendritic spines. The serum containing ZBPYR was prepared by seropharmacology. Reverse transcription and polymerase chain reaction (RT-PCR) was used to detect the expression of mRNA for SNK

SPAR

postsynaptic density protein 95 (PSD-95) and N-methyl-D-aspartate (NMDA) receptor subunits (NR1

NR2A and NR2B) in primary rat hippocampal neuron cultures after pretreatment with 10 μmol/L glutamate and ZBPYR serum. ZBPYR serum pretreatment resulted in a significant down-regulation of glutamate-induced SNK mRNA expression (P<0.05). Significant up-regulation was seen on the mRNA expression of SPAR and PSD-95 (P<0.05). All these changes were dose-dependent. The mRNA expression of NR1

NR2A and NR2B was down-regulated to different degrees (P<0.05). The mechanism of effect of ZBPYR on glutamate-induced excitotoxicity may be related to the regulation of SNK-SPAR signal pathway. ZBPYR may play a role in protecting and maintaining the normal morphology and structure of dendritic spines

which may be achieved by inhibiting the excessive activation of NMDA receptors.

Abstract

to explore the molecular mechanism of neuroprotection of Zibu Piyin Recipe (滋补脾阴方药

SPAR

postsynaptic density protein 95 (PSD-95) and N-methyl-D-aspartate (NMDA) receptor subunits (NR1

which may be achieved by inhibiting the excessive activation of NMDA receptors.

关键词

Alzheimer’s diseaseZibu Piyin Recipeserum-inducible kinasespine-associated Rap GTPase-activating proteinexcitotoxicitydendritic spine

Keywords

Alzheimer’s diseaseZibu Piyin Recipeserum-inducible kinasespine-associated Rap GTPase-activating proteinexcitotoxicitydendritic spine

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Sans N, Petralia RS, Wang YX, et al. A developmental change in NMDA receptor-associated proteins at hippocampal synapses. J Neurosci 2000;20:1260–1271.

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