Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro

Hui Liao; Linda K. Banbury; David N. Leach

doi:10.1007/s11655-008-0128-y

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Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro

OriginalPaper | Updated：2021-08-27

- Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro
- Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro
- 中国结合医学杂志（英文版） 2008年14卷第2期页码：128-131
- Affiliations：
  
  1. Shanxi Provincial People’s Hospital,Taiyuan,China
  2. Centre for Phytochemistry and Pharmacology, Southern Cross University, Lismore,Australia
- Author bio：
- Funds：
- DOI：10.1007/s11655-008-0128-y
  中图分类号：
- 纸质出版日期：2008，
  
  网络出版日期：2008-8-6，
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Liao, H., Banbury, L.K. & Leach, D.N. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro, ., Chin. J. Integr. Med. 14, 128–131 (2008). https://doi.org/10.1007/s11655-008-0128-y

Hui Liao, Linda K. Banbury, David N. Leach. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro[J]. Chinese Journal of Integrative Medicine, 2008,14(2):128-131.
Liao, H., Banbury, L.K. & Leach, D.N. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro, ., Chin. J. Integr. Med. 14, 128–131 (2008). https://doi.org/10.1007/s11655-008-0128-y DOI：

Hui Liao, Linda K. Banbury, David N. Leach. Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro[J]. Chinese Journal of Integrative Medicine, 2008,14(2):128-131. DOI： 10.1007/s11655-008-0128-y.

摘要

To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill (丹栀逍遥丸

DXP) on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC). The effects of DXP on nitric oxide (NO) production were tested by lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages using the Griess reaction. The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10

0.25 and 0.50 mg/mL dose-dependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820 μmoL Trolox equivalent/g

about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL (P<0.05

P<0.01). The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells

with the effect possibly related to its antioxidant activity and the inhibition of NO production.

Abstract

To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill (丹栀逍遥丸

0.25 and 0.50 mg/mL dose-dependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820 μmoL Trolox equivalent/g

about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL (P<0.05

P<0.01). The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells

with the effect possibly related to its antioxidant activity and the inhibition of NO production.

关键词

Danzhi Xiaoyao Pillbreast cancer MCF-7 cellsantioxidant activityoxygen radical absorbance capacityNitric Oxide

Keywords

Danzhi Xiaoyao Pillbreast cancer MCF-7 cellsantioxidant activityoxygen radical absorbance capacityNitric Oxide

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