Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein

Zheng-zhi Wu; Andrew C. J. Huang; Jean de Vellis; Ying-hong Li

doi:10.1007/s11655-008-0286-y

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Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein

OriginalPaper | Updated：2021-08-27

- Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein
- Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein
- 中国结合医学杂志（英文版） 2008年14卷第4期页码：286-292
- Affiliations：
  
  1. Shenzhen Institute of Integrated Traditional and Western Medicine,Shenzhen,China
  2. Department of Medicine, Division of Endocrinology, Larry Hillblom Islet Research Center, David Geffen School of Medicine, UCLA,Los Angeles,USA
  3. Department of Neurobiology, Mental Retardation Research Center, Neuropsychiatric Institute David Geffen School of Medicine at UCLA,Los Angeles,USA
- Author bio：
- Funds：
- DOI：10.1007/s11655-008-0286-y
  中图分类号：
- 纸质出版日期：2008，
  
  网络出版日期：2008-12-12，
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Wu, Zz., Huang, A.C.J., de Vellis, J. et al. Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein., Chin. J. Integr. Med. 14, 286–292 (2008). https://doi.org/10.1007/s11655-008-0286-y

Zheng-zhi Wu, Andrew C. J. Huang, Jean de Vellis, et al. Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein[J]. Chinese Journal of Integrative Medicine, 2008,14(4):286-292.
Wu, Zz., Huang, A.C.J., de Vellis, J. et al. Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein., Chin. J. Integr. Med. 14, 286–292 (2008). https://doi.org/10.1007/s11655-008-0286-y DOI：

Zheng-zhi Wu, Andrew C. J. Huang, Jean de Vellis, et al. Effect of Tiantai No.1 (天泰1号) on β-amyloid-induced neurotoxicity and NF-κ B and cAMP responsive element-binding protein[J]. Chinese Journal of Integrative Medicine, 2008,14(4):286-292. DOI： 10.1007/s11655-008-0286-y.

摘要

To investigate the effect and molecular mechanism of Tiantai No.1 (天泰1号)

a compound Chinese herbal preparation

for the prevention and reduction of neurotoxicity induced by beta-amyloid peptides (Abeta) in vitro and its effects on nuclear factor-κ B (NF-κ B) and cAMP responsive element-binding protein (CREB) pathways using the gene transfection technique. B104 neuronal cells were used to examine the effects of Tiantai No.1 on lowering the neurotoxicity induced by Abeta. The cells were pre-treated with Tiantai No.1 at doses of 50

100

150

or 200 μ g/mL respectively for 3 days and co-treated with Tiantai No.1 and beta-amyloid peptide1–40 (A β 1–40

10 μ mol/L) for 48 h or post-treated with Tiantai No.1 for 48 h after the cells were exposed to beta-amyloid peptides25–35 (A β 25–35) for 8 h. In gene transfection assays

cells were treated with Tiantai No.1 at 50 μ g/mL and 150 μ g/mL for 5 days or co-treated with Tiantai No.1 and A β 1–40 (5 μ mo/L) for 3 days after electroporation for the evaluation of NF-κ B and CREB expression. Pre-treating and co-treating B104 neuronal cells with Tiantai No.1 lowered the neurotoxicity induced by Abeta

and post-treating with Tiantai No.1 reduced or blocked B104 neuronal apoptotic death induced by Abeta (P<0.05

P<0.01). With a dose-dependent relationship

the same treatments increased the expression of NF-κ B or CREB in B104 neuronal cells (P<0.05

P<0.01). Meanwhile

Tiantai No.1 reduced A β -40 induced inhibition on NF-κ B expression (P<0.01). Tiantai No.1 can protect neurons against the neurotoxicity induced by Abeta. The neuroprotective mechanisms may be associated with the activation of NF-κ B and cAMP cellular signal pathways.

Abstract

To investigate the effect and molecular mechanism of Tiantai No.1 (天泰1号)

a compound Chinese herbal preparation

100

150

or 200 μ g/mL respectively for 3 days and co-treated with Tiantai No.1 and beta-amyloid peptide1–40 (A β 1–40

10 μ mol/L) for 48 h or post-treated with Tiantai No.1 for 48 h after the cells were exposed to beta-amyloid peptides25–35 (A β 25–35) for 8 h. In gene transfection assays

and post-treating with Tiantai No.1 reduced or blocked B104 neuronal apoptotic death induced by Abeta (P<0.05

P<0.01). With a dose-dependent relationship

the same treatments increased the expression of NF-κ B or CREB in B104 neuronal cells (P<0.05

P<0.01). Meanwhile

关键词

Alzheimer’s diseasebeta-amyloid peptideapoptosisnuclear factor-κ BcAMP responsive element-binding proteinTiantai No.1

Keywords

Alzheimer’s diseasebeta-amyloid peptideapoptosisnuclear factor-κ BcAMP responsive element-binding proteinTiantai No.1

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